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PMID: 10488231 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmc1 mutant of Arabidopsis.

The Plant cell ·Vol. 11 ·No. 9 ·1999-09-00 ·Pages 1623-34

Couteau F, Belzile F, Horlow C, Grandjean O, Vezon D, Doutriaux MP

Abstract

In yeast, the DMC1 gene is required for interhomolog recombination, which is an essential step for bivalent formation and the correct partition of chromosomes during meiosis I. By using a reverse genetics approach, we were able to identify a T-DNA insertion in AtDMC1, the Arabidopsis homolog of DMC1. Homozygotes for the AtDMC1 insertion failed to express AtDMC1, and their residual fertility was 1.5% that of the wild type. Complete fertility was restored in mutant plants when a wild-type copy of the AtDMC1 gene was reintroduced. Cytogenetical analysis points to a correlation of the sterility phenotype with severely disturbed chromosome behavior during both male and female meiosis. In this study, our data demonstrate that AtDMC1 function is crucial for meiosis in Arabidopsis. However, meiosis can be completed in the Arabidopsis dmc1 mutant, which is not the case for mouse or some yeast mutants.

MeSH Terms
Adenosine Triphosphatases Animals Arabidopsis/cytology,genetics,growth & development Arabidopsis Proteins Base Sequence Cell Cycle Proteins Chromosomes/genetics DNA Primers/genetics DNA-Binding Proteins/genetics Genes, Plant Genetic Complementation Test Meiosis/genetics Mice Mutagenesis, Insertional Mutation Nuclear Proteins Phenotype Phosphate-Binding Proteins Plant Proteins/genetics Rec A Recombinases
Chemicals
Arabidopsis Proteins Cell Cycle Proteins DNA Primers DNA-Binding Proteins Dmc1 protein, mouse Nuclear Proteins Phosphate-Binding Proteins Plant Proteins ATDMC1 protein, Arabidopsis Rec A Recombinases Adenosine Triphosphatases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Couteau F
Institut de Biotechnologie des Plantes, Bâtiment 630, Université Paris-Sud, 91405 Orsay, France. [email protected]
Belzile F
Horlow C
Grandjean O
Vezon D
Doutriaux M P
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.11.9.1623
Email
[email protected]
Published
1999-09-00
Pages
1623-34
Language
English
Region
England
NLM ID
9208688
PMCID
PMC144309
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.11.9.1623 PMC Full Text View journal details
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