The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation.
The new pPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322 bom site for mobilization from Escherichia coli to Agrobacterium, and the ColE1 and pVS1 plasmid origins for replication in E. coli and in Agrobacterium, respectively. Bacterial marker genes in the vectors confer resistance to chloramphenicol (pPZP100 series) or spectinomycin (pPZP200 series), allowing their use in Agrobacterium strains with different drug resistance markers. Plant marker genes in the binary vectors confer resistance to kanamycin or to gentamycin, and are adjacent to the left border (LB) of the transferred region. A lacZ alpha-peptide, with the pUC18 multiple cloning site (MCS), lies between the plant marker gene and the right border (RB). Since the RB is transferred first, drug resistance is obtained only if the passenger gene is present in the transgenic plants.
29 references, click to expand
-
The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing.Gene. 1988 Aug 15;68(1):139-49 PMID: 2851488
-
Agrobacterium and plant genetic engineering.Plant Mol Biol. 1992 May;19(1):15-38 PMID: 1600167
-
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
-
Expression of bacterial genes in plant cells.Proc Natl Acad Sci U S A. 1983 Aug;80(15):4803-7 PMID: 6308651
-
Development of plant promoter expression vectors and their use for analysis of differential activity of nopaline synthase promoter in transformed tobacco cells.Plant Physiol. 1986 May;81(1):86-91 PMID: 16664813
-
Chimeric genes as dominant selectable markers in plant cells.EMBO J. 1983;2(6):987-95 PMID: 16453464
-
Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants.Transgenic Res. 1992 Nov;1(6):285-97 PMID: 1338696
-
The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA.J Bacteriol. 1986 Dec;168(3):1291-301 PMID: 3782037
-
Kanamycin resistance as a selectable marker for plastid transformation in tobacco.Mol Gen Genet. 1993 Oct;241(1-2):49-56 PMID: 8232211
-
New plant binary vectors with selectable markers located proximal to the left T-DNA border.Plant Mol Biol. 1992 Dec;20(6):1195-7 PMID: 1463855
-
Transfection and transformation of Agrobacterium tumefaciens.Mol Gen Genet. 1978 Jul 11;163(2):181-7 PMID: 355847
-
A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. 1983.Biotechnology. 1992;24:367-70 PMID: 1422041
-
Facile transformation of Arabidopsis.Plant Cell Rep. 1991 Aug;10(5):235-9 PMID: 24221586
-
Binary Agrobacterium vectors for plant transformation.Nucleic Acids Res. 1984 Nov 26;12(22):8711-21 PMID: 6095209
-
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
-
Genetic and molecular characterization of the Pseudomonas plasmid pVS1.Plasmid. 1984 May;11(3):206-20 PMID: 6087391
-
Cloning vectors derived from the Pseudomonas plasmid pVS1.Gene. 1985;36(1-2):27-36 PMID: 2998935
-
The plasmid cloning vector pBR325 contains a 482 base-pair-long inverted duplication.Gene. 1981 Sep;14(4):289-99 PMID: 6271628
-
The pFF plasmids: cassettes utilising CaMV sequences for expression of foreign genes in plants.J Biotechnol. 1990 Jun;14 (3-4):333-44 PMID: 1369289
-
A new revision of the sequence of plasmid pBR322.Gene. 1988 Oct 30;70(2):399-403 PMID: 3063608
-
Gentamycin resistance in Nicotiana conferred by AAC(3)-I, a narrow substrate specificity acetyltransferase.Plant Mol Biol. 1991 Aug;17(2):301-3 PMID: 1650617
-
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.Gene. 1985;33(1):103-19 PMID: 2985470
-
A simple and general method for transferring genes into plants.Science. 1985 Mar 8;227(4691):1229-31 PMID: 17757866
-
Characterization of transferable plasmids from Shigella flexneri 2a that confer resistance to trimethoprim, streptomycin, and sulfonamides.Plasmid. 1986 Mar;15(2):119-31 PMID: 3517903
-
Nucleotide sequence analysis of a gene encoding a streptomycin/spectinomycin adenylyltransferase.Plasmid. 1985 Jan;13(1):17-30 PMID: 2986186
-
Short direct repeats flank the T-DNA on a nopaline Ti plasmid.Proc Natl Acad Sci U S A. 1982 Oct;79(20):6322-6 PMID: 16593241
-
Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA.J Mol Appl Genet. 1982;1(4):361-70 PMID: 7108407
-
Genetic analysis of Agrobacterium.Methods Enzymol. 1991;204:384-97 PMID: 1658565
-
Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumour-inducing plasmid.EMBO J. 1986 Jun;5(6):1137-42 PMID: 15966101
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
