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PMID: 10516084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Rescue of influenza A virus from recombinant DNA.

Journal of virology ·Vol. 73 ·No. 11 ·1999-11-00 ·Pages 9679-82

Fodor E, Devenish L, Engelhardt OG, Palese P, Brownlee GG, García-Sastre A

Abstract

We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.

MeSH Terms
Animals Cell Line DNA, Recombinant/genetics Genetic Techniques Humans Influenza A virus/genetics,physiology Plasmids/genetics RNA, Viral/metabolism Transcription, Genetic Transfection Viral Proteins/metabolism
Chemicals
DNA, Recombinant RNA, Viral Viral Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fodor E
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Devenish L
Engelhardt O G
Palese P
Brownlee G G
García-Sastre A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.11.9679-9682.1999
Published
1999-11-00
Pages
9679-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC113010
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.73.11.9679-9682.1999 PMC Full Text View journal details
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