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PMID: 10521393 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo.

Genes & development ·Vol. 13 ·No. 19 ·1999-10-01 ·Pages 2484-9

Komarnitsky PB, Michel B, Buratowski S

Abstract

Many questions remain concerning the role of TFIID TBP-associated factors (TAFs) in transcription, including whether TAFs are required at most or only a small subset of promoters. It was shown previously that three histone-like TAFs are broadly required for transcription, but interpretation of this observation is complicated because these proteins are components of both TFIID and the SAGA histone acetyltransferase complex. Here we show that mutations in the yeast TFIID-specific protein Taf40 lead to a general cessation of transcription, even in the presence of excess TBP, suggesting that the TFIID complex is required at most promoters in vivo.

MeSH Terms
DNA-Binding Proteins/genetics,metabolism Gene Expression Mutagenesis RNA Polymerase II/metabolism Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Temperature Transcription Factor TFIID Transcription Factors/genetics,metabolism Transcription Factors, TFII/metabolism Transcription, Genetic Yeasts/genetics
Chemicals
DNA-Binding Proteins Saccharomyces cerevisiae Proteins TAF11 protein, S cerevisiae TATA-Binding Protein Associated Factors TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors Transcription Factors, TFII RNA Polymerase II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Komarnitsky P B
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Michel B
Buratowski S
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41 references, click to expand
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.19.2484
Published
1999-10-01
Pages
2484-9
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC317065
Subset
IM
Grants
NIGMS NIH HHS · R01 GM046498 · United States
NIGMS NIH HHS · GM46498 · United States
External Links
PubMed source DOI: 10.1101/gad.13.19.2484 PMC Full Text View journal details
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