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PMID: 8170939 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction between the N-terminal domain of the 230-kDa subunit and the TATA box-binding subunit of TFIID negatively regulates TATA-box binding.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 9 ·1994-04-26 ·Pages 3520-4

Kokubo T, Yamashita S, Horikoshi M, Roeder RG, Nakatani Y

Abstract

Transcription initiation factor TFIID plays a central role in transcriptional regulation. Drosophila TFIID is a multimeric protein consisting of the TATA box-binding polypeptide (TBP) and a number of tightly associated polypeptides. Previously, the largest subunit of TFIID (p230) was cloned and demonstrated to inhibit the TATA-box binding of TBP in the absence of other subunits. Here we demonstrate that p230 contains at least two sites of interaction with TBP and that the N-terminal site mediates both strong physical interactions with TBP and inhibition of the TBP function. A detailed mutagenesis study shows that the inhibitory domain is indistinguishable from the strong TBP-binding domain, thus indicating that interaction of the p230 N-terminal region with TBP may directly control TATA-box binding.

MeSH Terms
Amino Acid Sequence Animals DNA-Binding Proteins/metabolism Drosophila melanogaster Macromolecular Substances Molecular Sequence Data Protein Binding Sequence Deletion TATA Box TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors/metabolism
Chemicals
DNA-Binding Proteins Macromolecular Substances TATA-Box Binding Protein Transcription Factor TFIID Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kokubo T
National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
Yamashita S
Horikoshi M
Roeder R G
Nakatani Y
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.9.3520
Published
1994-04-26
Pages
3520-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43611
Subset
IM
Grants
NIAID NIH HHS · AI27397 · United States
NCI NIH HHS · CA42567 · United States
NIGMS NIH HHS · GM45258 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.9.3520 PMC Full Text View journal details
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