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PMID: 8327460 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Drosophila 110-kDa transcription factor TFIID subunit directly interacts with the N-terminal region of the 230-kDa subunit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 13 ·1993-07-01 ·Pages 5896-900

Kokubo T, Gong DW, Roeder RG, Horikoshi M, Nakatani Y

Abstract

Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in transcriptional regulation by facilitating promoter responses to various activators. cDNAs encoding the 110-kDa subunit of Drosophila TFIID (p110) were isolated with a degenerate oligodeoxynucleotide probe based on an amino acid sequence of the purified protein. The entire cDNA sequence contains an open reading frame encoding a 921-amino acid polypeptide with a calculated molecular mass of 99,337 Da. The recombinant protein expressed in Sf9 cells via a baculovirus vector interacts directly with the 230-kDa subunit of TFIID (p230). Together with the previous observation that the TATA box-binding subunit of TFIID (TFIID tau or TBP) interacts directly with only p230 among the TFIID subunits, this result suggests that p110 forms a complex with TFIID tau via p230. A binding study using various p230 mutants indicated that both p110 and TFIID tau interact with the N-terminal 352-amino acid portion of p230, suggesting a functional communication between p110 and TFIID tau via p230 interactions.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/chemistry Drosophila Molecular Sequence Data Molecular Weight Peptide Fragments/chemistry,genetics,physiology Rabbits Transcription Factor TFIID Transcription Factors/chemistry,genetics,physiology
Chemicals
Peptide Fragments Transcription Factor TFIID Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kokubo T
National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.
Gong D W
Roeder R G
Horikoshi M
Nakatani Y
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.13.5896
Published
1993-07-01
Pages
5896-900
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46833
Subset
IM
Grants
NIAID NIH HHS · AI27397 · United States
NCI NIH HHS · CA42567 · United States
NIGMS NIH HHS · GM45258 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.13.5896 PMC Full Text View journal details
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