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PMID: 1828892 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 12 ·1991-06-15 ·Pages 5124-8

Horikoshi N, Maguire K, Kralli A, Maldonado E, Reinberg D, Weinmann R

Abstract

Adenovirus E1A has long been known to activate/repress cellular and viral transcription. The transcriptional activity of nuclear extracts was depleted after chromatography on immobilized E1A protein columns that specifically retained the transcription factor (TF) IID. Stronger direct interactions between E1A and human TFIID than between E1A and yeast TFIID suggest that the unique sequences of the human protein may be involved. We have demonstrated that this interaction occurs directly between bacterially produced E1A and bacterially produced human TFIID in a protein blot assay. We propose that E1A protein may transduce regulatory signals from upstream activators to basal elements of the transcriptional machinery by contacting TFIID.

MeSH Terms
Adenovirus Early Proteins Antigens, Viral, Tumor/immunology Blotting, Western Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Humans Oncogene Proteins, Viral/metabolism Saccharomyces cerevisiae/metabolism TATA Box Transcription Factor TFIID Transcription Factors/metabolism Transcription, Genetic
Chemicals
Adenovirus Early Proteins Antigens, Viral, Tumor Oncogene Proteins, Viral Transcription Factor TFIID Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Horikoshi N
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Maguire K
Kralli A
Maldonado E
Reinberg D
Weinmann R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.12.5124
Published
1991-06-15
Pages
5124-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51824
Subset
IM
Grants
NCI NIH HHS · CA08338 · United States
NCI NIH HHS · CA10815 · United States
NCI NIH HHS · CA44466 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.12.5124 PMC Full Text 免费全文 View journal details
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