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PMID: 2431282 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Association of adenovirus early-region 1A proteins with cellular polypeptides.

Molecular and cellular biology ·Vol. 6 ·No. 5 ·1986-05-00 ·Pages 1579-89

Harlow E, Whyte P, Franza BR, Schley C

Abstract

Extracts from adenovirus-transformed human 293 cells were immunoprecipitated with monoclonal antibodies specific for the early-region 1A (E1A) proteins. In addition to the E1A polypeptides, these antibodies precipitated a series of proteins with relative molecular weights of 28,000, 40,000, 50,000, 60,000, 80,000, 90,000, 110,000, 130,000, and 300,000. The two most abundant of these polypeptides are the 110,000-molecular-weight protein (110K protein) and 300K protein. Three experimental approaches have suggested that the 110K and 300K polypeptides are precipitated because they form stable complexes with the E1A proteins. The 110K and 300K polypeptides do not share epitopes with the E1A proteins, they copurify with a subset of the E1A proteins, and they bind to the E1A proteins following mixing in vitro. The 110K and 300K polypeptides are not adenoviral proteins, but are encoded by cellular DNA. Both the 12S and the 13S E1A proteins bind to the 110K and 300K species, and these complexes are found in adenovirus-transformed and -infected cells.

MeSH Terms
Adenovirus Early Proteins Adenoviruses, Human/genetics Antibodies, Monoclonal/analysis Cell Line Cell Transformation, Neoplastic Electrophoresis, Polyacrylamide Gel Epitopes/analysis Genes Genes, Viral HeLa Cells Humans Molecular Weight Oncogene Proteins, Viral/analysis,genetics Viral Proteins/analysis,genetics
Chemicals
Adenovirus Early Proteins Antibodies, Monoclonal Epitopes Oncogene Proteins, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Harlow E
Whyte P
Franza B R
Schley C
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.5.1579-1589.1986
Published
1986-05-00
Pages
1579-89
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367684
Subset
IM
Grants
NCI NIH HHS · CA13106 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.5.1579-1589.1986 PMC Full Text 免费全文 View journal details
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