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PMID: 10545472 Published · ppublish English Journal Article

DNA variation in the basic chitinase locus (ChiB) region of the wild plant Arabidopsis thaliana.

Genetics ·Vol. 153 ·No. 3 ·1999-11-00 ·Pages 1445-53

Kawabe A, Miyashita NT

Abstract

Nucleotide variation in a 2.2-kbp region of basic chitinase (ChiB) locus in 17 ecotypes of Arabidopsis thaliana was compared with previously investigated regions to investigate genetic mechanisms acting on DNA polymorphism. In the ChiB region, dimorphic DNA variation was detected, as in the Adh and ChiA regions. Nucleotide diversity (pi) of the entire region was 0.0091, which was similar to those of the two other regions. About half of polymorphic sites (37/87) in the ChiB region were observed in only two ecotypes. Tajima's D was negative but not significantly, while Fu and Li's D* was positive. Neither McDonald-Kreitman nor Hudson, Kreitman, Aguadé tests showed a significant result, indicating that these loci were under similar evolutionary mechanisms before and after speciation. Linkage disequilibria were observed within the three regions because of dimorphic polymorphisms. Interlocus linkage disequilibrium was not detected between the Adh and the two chitinase regions, but was observed between the ChiA and ChiB regions. This could be due to epistatic interaction between the two chitinase loci, which are located on different chromosomes.

MeSH Terms
Alcohol Dehydrogenase/genetics Arabidopsis/genetics Base Sequence Chitinases/genetics DNA, Plant/chemistry,genetics Evolution, Molecular Genetic Variation Linkage Disequilibrium Molecular Sequence Data Phylogeny Plant Proteins/genetics Polymorphism, Genetic
Chemicals
DNA, Plant Plant Proteins Alcohol Dehydrogenase Chitinases PLC-B protein, Phytolacca americana
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kawabe A
Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan.
Miyashita N T
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/153.3.1445
Published
1999-11-00
Pages
1445-53
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1460836
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/153.3.1445 PMC Full Text View journal details
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