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PMID: 1054839 Published · ppublish English Journal Article

Isolation of the bacteriophage lambda A-gene protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 2 ·1975-02-00 ·Pages 581-5

Becker A, Gold M

Abstract

An in vitro assay for measuring the activity of the phage lambda A-gene product has been developed. The assay is based on the observation that A-donor extracts complement A minus extracts for packaging of exogenous immature lambda DNA into phage particles. A partial purification of the A-gene product activity using this assay is presented. A method is suggested by which this A protein-dependent in vitro system might be manipulated to analyze the mechanism of reforming the lambda cohesive termini during chromosome assimilation into phage precursors.

MeSH Terms
Centrifugation, Density Gradient Chromatography, Ion Exchange Coliphages/analysis DNA Viruses/analysis DNA, Viral/metabolism Genes Genotype Viral Proteins/biosynthesis,isolation & purification
Chemicals
DNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Becker A
Gold M
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.2.581
Published
1975-02-00
Pages
581-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432357
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.2.581 PMC Full Text View journal details
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