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PMID: 1057169 Published · ppublish English Journal Article

Cooperative activation of action potential Na+ ionophore by neurotoxins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1782-6

Catterall WA

Abstract

Four neurotoxins that activate the action potential Na+ ionophore of electrically excitable neuroblastoma cells interact with two distinct classes of sites, one specific for the alkaloids veratridine, batrachotoxin, and aconitine, and the second specific for scorpion toxin. Positive heterotropic cooperativity is observed between toxins bound at these two classes of sites. Tetrodotoxin is a noncompetitive inhibitor of activation by each of these toxins (KI = 4-8 nM). These results suggest the existence of three functionally separable components of the action potential Na+ ionophore: two regulatroy components, which bind activating neurotoxins and interact allosterically in controlling the activity of a third ion-transport component, which binds tetrodotoxin.

MeSH Terms
Aconitine/pharmacology Action Potentials/drug effects Alkaloids/pharmacology Animals Batrachotoxins/pharmacology Biological Transport Cell Line Cell Membrane/drug effects,metabolism Clone Cells Kinetics Neuroblastoma Peptides/pharmacology Scorpions Sodium/metabolism Structure-Activity Relationship Tetrodotoxin/pharmacology Toxins, Biological/pharmacology Venoms/pharmacology Veratridine/pharmacology
Chemicals
Alkaloids Batrachotoxins Peptides Toxins, Biological Venoms Tetrodotoxin Veratridine Sodium Aconitine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Catterall W A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.5.1782
Published
1975-05-00
Pages
1782-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432630
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.5.1782 PMC Full Text View journal details
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