Sodium uptake associated with activation of action potential ionophores of cultured neuroblastoma and muscle cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 12 ·1973-12-00 ·Pages 3759-63

Catterall WA, Nirenberg M

Abstract

Veratridine, an activator of action potential Na(+) ionophores, stimulated passive Na(+) uptake by electrically excitable neuroblastoma and muscle cells but had no effect on clonal cell lines defective in Na(+)-ionophore activity. Veratridine-dependent Na(+) uptake was completely inhibited by tetrodotoxin, a specific inhibitor of the action potential Na(+) ionophore. Half-maximal inhibition was obtained with 11 nM tetrodotoxin. Thus, veratridinedependent Na(+) uptake provides a specific and convenient means of assaying populations of cultured cells for action potential Na(+)-ionophore activity.

MeSH Terms

Action Potentials/drug effects Animals Biological Transport/drug effects Cell Membrane Permeability Clone Cells Glioma Hybrid Cells L Cells Mice Muscles/metabolism Neuroblastoma Neurons/metabolism Ouabain/pharmacology Radioisotopes Rats Sodium/metabolism Sodium Isotopes Stimulation, Chemical Tetrodotoxin/pharmacology Thymidine/metabolism Tritium

Chemicals

Radioisotopes Sodium Isotopes Tritium Tetrodotoxin Ouabain Sodium Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Catterall W A

Nirenberg M

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28 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.70.12.3759

Published

1973-12-00

Pages

3759-63

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC427322

Subset

External Links

PubMed source DOI: 10.1073/pnas.70.12.3759 PMC Full Text View journal details

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Sodium uptake associated with activation of action potential ionophores of cultured neuroblastoma and muscle cells.

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