Home LiteratureArticle Details
PMID: 1058468 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Antigen mobility in membranes and complement-medical immune attack.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 7 ·1975-07-00 ·Pages 2483-7

Humphires GM, McConnell HM

Abstract

The complement fixing activity of liposomes containing cholesterol, dimyristoylphosphatidylcholine (or dipalmitoylphosphatidylcholine), and 3 mol % of cardiolipin has been studied as a function of cholesterol concentration by use of human syphilitic serum containing cardiolipin-specific (Wasserman) antibodies. It is found that complement fixation increases rapidly for cholesterol concentrations above 35 mol %. Spin label studies have been used to study the incorporation of cardiolipin in the relatively rigid phase of binary mixtures of cholesterol and dimyristolphosphatidycholine (or dipalmitoylphosphatidylcholine). It is concluded that cardiolipin is included in such a phase of these lipids for cholesterol concentrations above 35 mol %. These results indicate that a relatively rigid lateral distribution of this monovalent antigen in the plane of the membrane facilitates complement fixation and concomitant complement-mediated membrane damage.

MeSH Terms
Animals Antigens Binding Sites Cardiolipins/immunology Cholesterol Chromatography, Thin Layer Complement Fixation Tests Complement System Proteins Erythrocytes/immunology Humans Kinetics Membranes, Artificial Models, Biological Phosphatidylcholines Rabbits/immunology Sheep/immunology Syphilis/immunology Temperature
Chemicals
Antigens Cardiolipins Membranes, Artificial Phosphatidylcholines Complement System Proteins Cholesterol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Humphires G M
McConnell H M
References (17)
17 references, click to expand
  1. Microviscosity and order in the hydrocarbon region of phospholipid and phospholipid-cholesterol dispersions determined with fluorescent probes.
    Biochemistry. 1973 Jan 30;12(3):521-8 PMID: 4683495
  2. Glycophorin in lipid bilayers.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4653-7 PMID: 4373724
  3. Anionic sites of human erythrocyte membranes. II. Antispectrin-induced transmembrane aggregation of the binding sites for positively charged colloidal particles.
    J Cell Biol. 1973 Nov;59(2 Pt 1):395-406 PMID: 4141707
  4. Active vs. passive sensitization of liposomes toward antibody and complement by dinitrophenylated derivatives of phosphatidylethanolamine.
    Biochemistry. 1972 Oct 24;11(22):4085-94 PMID: 4563929
  5. Lateral phase separation in phospholipid membranes.
    Biochemistry. 1973 Jun 5;12(12):2351-60 PMID: 4351059
  6. Lateral phase separations in binary lipid mixtures: correlation between spin label and freeze-fracture electron microscopic studies.
    Biochim Biophys Acta. 1974 Sep 6;363(2):151-8 PMID: 4371497
  7. The planar organization of lecithin-cholesterol bilayers.
    J Biol Chem. 1972 Jun 10;247(11):3694-7 PMID: 5030638
  8. Immune lysis of liposomes and erythrocyte ghosts loaded with spin label.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1691-4 PMID: 4365759
  9. Effect of immunoglobulin class and affinity on the initiation of complement-dependent damage to liposomal model membranes sensitized with dinitrophenylated phospholipids.
    Biochemistry. 1973 Sep 25;12(20):4003-11 PMID: 4795683
  10. Molecular motion in spin-labeled phospholipids and membranes.
    J Am Chem Soc. 1971 Jan 27;93(2):314-26 PMID: 5541516
  11. Receptor mobility and receptor-cytoplasmic interactions in lymphocytes.
    Proc Natl Acad Sci U S A. 1973 May;70(5):1442-6 PMID: 4514314
  12. Calorimetric investigation of the influence of cholesterol on the transition properties of bilayers formed from synthetic L- -lecithins in aqueous suspension.
    J Biol Chem. 1972 Jun 10;247(11):3697-700 PMID: 5030639
  13. Deuteron resonance: A novel approach to the study of hydrocarbon chain mobility in membrane systems.
    FEBS Lett. 1971 Aug 1;16(2):102-104 PMID: 11945913
  14. Intramembrane particle aggregation in erythrocyte ghosts. I. The effects of protein removal.
    J Cell Biol. 1974 Dec;63(3):1018-36 PMID: 4215819
  15. Lateral phase separations in binary mixtures of cholesterol and phospholipids.
    Biochem Biophys Res Commun. 1973 Jul 17;53(2):446-51 PMID: 4352068
  16. An interacting spin label study of the fluidizing and condensing effects of cholesterol on lecithin bilayers.
    Biochim Biophys Acta. 1973 Mar 16;298(2):133-44 PMID: 4352487
  17. Properties of 19 S antibodies in complement fixation. I. Temperature dependence and role of antigen structure.
    J Immunol. 1968 Jan;100(1):7-14 PMID: 5635627
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.7.2483
Published
1975-07-00
Pages
2483-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432791
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.7.2483 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2