Home LiteratureArticle Details
PMID: 1059086 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Electrophoretic patterns of deadenylylated chorion and globin mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 8 ·1975-08-00 ·Pages 2959-63

Vournakis JN, Efstratiadis A, Kafatos FC

Abstract

Treatment of silkmoth chorion mRNAs with calf thymus RNase H (EC 3.1.4.34; RNA-DNA-hybrid ribonucleotidohydrolase) in the presence of oligo(dT) specifically and effectively removes the 3'-terminal poly(A) sequences. Excision of non-poly(A) fragments cannot be detected. Under these conditions, RNase H leads to increased electrophoretic homogeneity of rabbit globin mRNA, presumably as a result of removal of poly(A) sequences that are inherently variable in length. Treatment with RNase H converts the three diffuse zones of messages for the several chorion proteins into multiple sharp bands.

MeSH Terms
Adenosine Monophosphate/analysis Animals Base Sequence Chorion/analysis Electrophoresis, Polyacrylamide Gel Extraembryonic Membranes/analysis Globins/biosynthesis Moths/analysis RNA, Messenger/analysis Rabbits Reticulocytes/analysis Ribonucleases
Chemicals
RNA, Messenger Adenosine Monophosphate Globins Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vournakis J N
Efstratiadis A
Kafatos F C
References (20)
20 references, click to expand
  1. The molecular weight of rabbit globin messenger RNA's.
    FEBS Lett. 1973 Mar 15;30(3):301-4 PMID: 4700749
  2. Translation of mouse globin messenger ribonucleic acid from which the poly(adenylic acid) sequence has been removed.
    Biochemistry. 1974 Feb 12;13(4):703-7 PMID: 4129862
  3. Size heterogeneity of polyadenylate sequences in mouse globin messenger RNA.
    J Mol Biol. 1974 Jun 25;86(2):363-71 PMID: 4415788
  4. The separation of alpha- and beta-rabbit globin mRNA by polyacrylamide gel electrophoresis.
    FEBS Lett. 1974 Oct 15;47(2):343-7 PMID: 4430361
  5. Translational properties of rabbit globin mRNA after specific removal of poly(A) with ribonuclease H.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4635-9 PMID: 4531006
  6. In vitro translation of polyadenylic acid-free rabbit globin messenger RNA.
    J Mol Biol. 1974 Sep 5;88(1):233-45 PMID: 4613842
  7. Sequence at the 3' end of globin mRNA shows homology with immunoglobulin light chain mRNA.
    Nature. 1974 Nov 29;252(5482):359-62 PMID: 4139664
  8. Purification of a family of specific messenger ribonucleic acids from moth follicular cells.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3764-8 PMID: 4521202
  9. Organization, transcription, and regulation in the animal genome.
    Q Rev Biol. 1973 Dec;48(4):565-613 PMID: 4592421
  10. Full length and discrete partial reverse transcripts of globin and chorion mRNAs.
    Cell. 1975 Apr;4(4):367-78 PMID: 47272
  11. Degradation of DNA RNA hybrids by ribonuclease H and DNA polymerases of cellular and viral origin.
    Proc Natl Acad Sci U S A. 1972 Nov;69(11):3360-4 PMID: 4343966
  12. The nucleotide sequence of the lac operator.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4 PMID: 4587255
  13. The radioiodination of ribopolymers for use in hybridizational and molecular analyses.
    J Biol Chem. 1974 Apr 10;249(7):2143-50 PMID: 4594497
  14. Purification and properties of ribonuclease H of calf thymus.
    Proc Natl Acad Sci U S A. 1973 Jul;70(7):1959-63 PMID: 4516197
  15. Short polyadenylic acid sequences in insect chorion messenger RNA.
    Cell. 1974 Nov;3(3):265-73 PMID: 4434474
  16. A comparative study of eggshell proteins in lepidoptera.
    J Supramol Struct. 1972;1(1):60-5 PMID: 4650443
  17. Specific protein synthesis in cellular differentiation. Production of eggshell proteins by silkmoth follicular cells.
    J Cell Biol. 1972 Dec;55(3):653-80 PMID: 4656706
  18. Iodination of nucleic acids in vitro.
    Biochemistry. 1971 May 25;10(11):1993-2000 PMID: 4935105
  19. Possible relationship of poly(A) shortening to mRNA turnover.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1077-81 PMID: 1055367
  20. Individual histone messenger RNAs: identification by template activity.
    Cell. 1975 Mar;4(3):239-48 PMID: 1122555
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.8.2959
Published
1975-08-00
Pages
2959-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432898
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.8.2959 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2