Home LiteratureArticle Details
PMID: 1060124 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Adenovirus-2 mRNA is transcribed as part of a high-molecular-weight precursor RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4445-9

Bachenheimer S, Darnell JE

Abstract

The order of transcription and the length of nascent RNA transcripts from adenovirus-2 (Ad-2) DNA in the nucleus of infected cells has been deduced by labeling the growing RNA chains in vivo for a very brief period, separating the RNA on the basis of size, and hybridizine to the ordered EcoRI restriction endonuclease fragments derived from Ad-2 DNA. The majority of the virus-specific RNA molecules are synthesized as very high-molecular-weight units beginning at a common point at least 25-30,000 base pairs from one end of the Ad-2 DNA. These molecules can be reduced in size without further RNA synthesis. The experiments indicate the obligatory origin of Ad-2 mRNA from a high-molecular-weight precursor molecule.

MeSH Terms
Adenoviridae/metabolism HeLa Cells Models, Biological Molecular Weight Nucleic Acid Precursors/biosynthesis RNA, Messenger/biosynthesis RNA, Viral/analysis,biosynthesis Time Factors Transcription, Genetic
Chemicals
Nucleic Acid Precursors RNA, Messenger RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bachenheimer S
Darnell J E
References (24)
24 references, click to expand
  1. Effect of actinomycin D on cellular nucleic acid synthesis and virus production.
    Science. 1961 Aug 25;134(3478):556-7 PMID: 13740412
  2. Assembly of the peptide chains of hemoglobin.
    Proc Natl Acad Sci U S A. 1961 Mar 15;47:247-61 PMID: 13723017
  3. RNA synthesis and processing in adenovirus-infected cells.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:447-56 PMID: 1057473
  4. Mapping of late adenovirus genes by cell-free translation of RNA selected by hybridization to specific DNA fragments.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1344-8 PMID: 1093178
  5. Demonstration of globin messenger sequences in giant nuclear precursors of messenger RNA of avian erythroblasts.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1122-6 PMID: 4123930
  6. Specific origin in SV40 DNA replication.
    Nat New Biol. 1972 Apr 19;236(68):200-2 PMID: 4336209
  7. New method of studying the precursor-product relationship between high molecular weight RNA and messenger RNA.
    Nat New Biol. 1972 Apr 12;236(67):172-4 PMID: 4503731
  8. tRNA synthesis in HeLa cells: a precursor to tRNA and the effects of methionine starvation on tRNA synthesis.
    J Mol Biol. 1969 May 28;42(1):43-56 PMID: 4185508
  9. The origin of mRNA and the structure of the mammalian chromosome.
    Harvey Lect. 1973-1974;(69):1-47 PMID: 4376811
  10. Secondary structure in heterogeneous nuclear RNA: involvement of regions from repeated DNA sites.
    J Mol Biol. 1974 Jan 25;82(3):361-70 PMID: 4361749
  11. SV40-specific RNA in the nucleus and polyribosomes of transformed cells.
    Proc Natl Acad Sci U S A. 1970 Apr;65(4):1089-96 PMID: 4314900
  12. T7 early RNAs and Escherichia coli ribosomal RNAs are cut from large precursor RNAs in vivo by ribonuclease 3.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3296-3300 PMID: 4587248
  13. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):200-4 PMID: 4567334
  14. Adenovirus messenger RNA in mammalian cells: failure of polyribosome association in the absence of nuclear cleavage.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1578-82 PMID: 4504372
  15. Nucleotide sequences of the 5' and 3' termini of bacteriophage T7 early messenger RNAs synthesized in vivo: evidence for sequence specificity in RNA processing.
    J Mol Biol. 1974 Nov 15;89(4):767-76 PMID: 4449132
  16. Processing of adenovirus specific nuclear RNA during virus replication.
    Virology. 1972 Oct;50(1):27-34 PMID: 5081848
  17. Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection.
    J Virol. 1972 Nov;10(5):909-19 PMID: 4636395
  18. Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2806-9 PMID: 5315962
  19. Biochemical studies on adenovirus multiplication, XIX. Resolution of late viral RNA species in the nucleus and cytoplasm.
    Proc Natl Acad Sci U S A. 1971 Mar;68(3):557-60 PMID: 5276761
  20. RNA processing and the control of gene expression.
    Brookhaven Symp Biol. 1975 Jul;(26):240-66 PMID: 1104093
  21. Methylated, blocked 5 termini in HeLa cell mRNA.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1904-8 PMID: 1057180
  22. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:457-74 PMID: 1057474
  23. Intracellular location and molecular characteristics of tumour cell transfer RNA precursors.
    J Mol Biol. 1969 Jan 14;39(1):113-24 PMID: 5406273
  24. RNA metabolism in the HeLa cell nucleus.
    J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.11.4445
Published
1975-11-00
Pages
4445-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388738
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.11.4445 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2