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PMID: 10634904 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The late developmental pattern of Mu transposon excision is conferred by a cauliflower mosaic virus 35S -driven MURA cDNA in transgenic maize.

The Plant cell ·Vol. 12 ·No. 1 ·2000-01-00 ·Pages 5-21

Raizada MN, Walbot V

Abstract

The MuDR element responsible for Mutator activities in maize encodes two genes, mudrA and mudrB. Each encodes multiple transcripts hypothesized to regulate, directly or indirectly, the unique late timing and switch in transposition mechanism during maize development. mudrA, which encodes the MURA transposase, is unstable in bacterial plasmids, a technical problem solved by using phage M13 as a vector to prepare DNA for biolistic transformation. In transgenic maize, a single 2.7-kb mudrA cDNA predicted to encode an 823-amino acid protein is sufficient to catalyze late somatic excisions, despite removal of the native promoter, alternative transcription start sites, known introns, polymorphic 5' and 3' untranslated sequences, and the mudrB gene. These results suggest that post-translational regulation confers Mu excision timing. The transgene is active in lines containing silencing MuDR elements. This suggests that endogenous MuDR transposons do not measurably immunize the host against expression of a homologous transgene.

MeSH Terms
Bacteriophage M13/genetics Base Sequence Caulimovirus/genetics Cell Line DNA Primers/genetics DNA Transposable Elements/genetics DNA, Complementary/genetics DNA, Plant/genetics Escherichia coli/genetics Genes, Plant Plants, Genetically Modified Plasmids/genetics Zea mays/genetics,growth & development,virology
Chemicals
DNA Primers DNA Transposable Elements DNA, Complementary DNA, Plant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Raizada M N
Department of Biological Sciences, Stanford University, Stanford, CA 94305-5020, USA.
Walbot V
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.12.1.5
Published
2000-01-00
Pages
5-21
Language
English
Region
England
NLM ID
9208688
PMCID
PMC140211
Subset
IM
Grants
NIGMS NIH HHS · R01 GM049681 · United States
NIGMS NIH HHS · GM43681 · United States
External Links
PubMed source DOI: 10.1105/tpc.12.1.5 PMC Full Text View journal details
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