Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus.

The EMBO journal ·Vol. 6 ·No. 9 ·1987-09-00 ·Pages 2519-23

Thompson CJ, Movva NR, Tizard R, Crameri R, Davies JE, Lauwereys M, Botterman J

Abstract

A gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. The bar gene product was purified and shown to be a modifying enzyme which acetylates phosphinothricin or demethylphosphinothricin but not bialaphos or glutamate. The bar gene was subcloned and its nucleotide sequence was determined. Interspecific transfer of this Streptomyces gene into Escherichia coli showed that it could be used as a selectable marker in other bacteria. In the accompanying paper, bar has been used to engineer herbicide-resistant plants.

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Thompson C J

Biogen S. A., 46 Route des Acacias, CH-1227 Geneva, Switzerland.

Movva N R

Tizard R

Crameri R

Davies J E

Lauwereys M

Botterman J

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1987-09-00

Pages

2519-23

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553668

External Links

PubMed source PMC Full Text View journal details

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Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus.

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