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PMID: 2992952 Published · ppublish English Journal Article

Resistance, regulatory and production genes for the antibiotic methylenomycin are clustered.

The EMBO journal ·Vol. 4 ·No. 7 ·1985-07-00 ·Pages 1893-7

Chater KF, Bruton CJ

Abstract

At least 17 kb of DNA from the large unisolatable Streptomyces coelicolor A3(2) plasmid SCP1 are concerned with methylenomycin biosynthesis. Mutational cloning analysis, using insert-directed integration of att site deleted phage vectors into an SCP1-containing host, provided evidence of two large transcription units, of at least 6.6 kb and 9.5 kb. At the leftmost apparent end of the larger (left-hand) transcription unit is a region apparently involved in negative regulation of methylenomycin biosynthesis: when fragments from this region were used to direct phage integration, marked overproduction of methylenomycin resulted. The methylenomycin resistance determinant is located at the rightmost end of this same transcription unit. Hybridisation analysis with 13 kb of the cloned mmy region showed that it was closely similar to a segment of pSV1, a plasmid that specifies methylenomycin biosynthesis in S. violaceus-ruber SANK 95570.

MeSH Terms
Anti-Bacterial Agents/biosynthesis Base Sequence Cloning, Molecular DNA Restriction Enzymes Drug Resistance, Microbial Genes Genes, Bacterial Genes, Regulator Genetic Linkage Mutation Peptides R Factors Streptomyces/genetics
Chemicals
Anti-Bacterial Agents Peptides DNA Restriction Enzymes methylenomycin A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chater K F
Bruton C J
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22 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-07-00
Pages
1893-7
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554433
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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