Home LiteratureArticle Details

PMID: 6593562 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Coupled transcription--translation in extracts of Streptomyces lividans.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 39-43

Thompson J, Rae S, Cundliffe E

Abstract

A coupled transcription-translation system has been developed using extracts of Streptomyces lividans. Analysis by polyacrylamide gel electrophoresis revealed that proteins of the expected sizes were produced in systems programmed by pBR 322 or the Streptomyces plasmid pIJ 350. Also, linear forms of these plasmids retained template activity with no apparent loss of fidelity.

MeSH Terms

Bacterial Proteins/genetics,isolation & purification Electrophoresis, Polyacrylamide Gel Kinetics Molecular Weight Plasmids Protein Biosynthesis Streptomyces/genetics Transcription, Genetic

Chemicals

Bacterial Proteins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Thompson J

Rae S

Cundliffe E

References (15)

15 references, click to expand

A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces.
Nature. 1980 Apr 10;284(5756):526-31 PMID: 7366721
pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.
Mol Gen Genet. 1982;185(2):223-8 PMID: 6283316
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Plasmid-directed expression of Staphylococcus aureus beta-lactamase by Bacillus subtilis in vitro.
J Biol Chem. 1981 Nov 10;256(21):11272-83 PMID: 6270148
Identification of gene products programmed by restriction endonuclease DNA fragments using an E. coli in vitro system.
Nucleic Acids Res. 1981 Sep 25;9(18):4459-74 PMID: 6272207
DNA cloning in Streptomyces: resistance genes from antibiotic-producing species.
Nature. 1980 Jul 31;286(5772):525-7 PMID: 6250070
Cloning and expression in streptomyces lividans of antibiotic resistance genes derived from Escherichia coli.
J Bacteriol. 1981 Apr;146(1):360-8 PMID: 7012122
Biochemical characterization of resistance determinants cloned from antibiotic-producing streptomycetes.
J Bacteriol. 1982 Aug;151(2):678-85 PMID: 6284707
Revised sequence of the tetracycline-resistance gene of pBR322.
Gene. 1983 May-Jun;22(2-3):277-80 PMID: 6307828
Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.
J Gen Microbiol. 1983 Jul;129(7):2257-69 PMID: 6631413
DNA cloning in Streptomyces: a bifunctional replicon comprising pBR322 inserted into a Streptomyces phage.
Nature. 1980 Jul 31;286(5772):527-9 PMID: 6250071
In vitro synthesis of ribosomal proteins directed by Escherichia coli DNA.
Proc Natl Acad Sci U S A. 1974 Feb;71(2):446-50 PMID: 4205593
DNA-directed cell-free protein-synthesizing system of Bacillus subtilis.
Biochim Biophys Acta. 1979 Aug 29;564(1):162-71 PMID: 93969
Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction.
Gene. 1982 Nov;20(1):51-62 PMID: 6298066
Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans.
Mol Gen Genet. 1982;187(2):265-77 PMID: 6294463

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00332721

Published

1984-00-00

Pages

39-43

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00332721

Analysis Services

Coupled transcription--translation in extracts of Streptomyces lividans.

Contact

WeChat Official Account