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PMID: 106366 Published · ppublish English Journal Article

The role of the guanine insertion enzyme in O-biosynthesis in Drosophila melanogaster.

Nucleic acids research ·Vol. 5 ·No. 12 ·1978-12-00 ·Pages 4865-76

McKinnon RD, Wosnick MA, White BN

Abstract

Drosophila tRNA can be guanylated by a crude enzyme from rabbit reticulocytes. Guanylating activity is also present in crude extracts of adult Drosophila. A major product of this reaction as well as several minor ones were resolved by RPC-5 chromatography. The main substrate of both the Drosophila and rabbit reticulocyte enzymes was the non-Q-containing aspartic acid tRNA, tRNA2gammaAsp. The QU-lacking (gamma) forms of asparagine, histidine and tyrosine tRNAs were also substrates and gave rise to the minor products of the reaction. In contrast, the Q- or Q*-containing (delta) forms of these tRNAs appear not to be substrates. The evidence strongly suggests that the guanyating enzyme is involved in Q biosynthesis and would be better termed a guanine replacement or pre-Q insertion enzyme.

MeSH Terms
Animals Chromatography, High Pressure Liquid Drosophila melanogaster/metabolism Guanine Guanosine/analogs & derivatives,biosynthesis,metabolism RNA, Transfer/biosynthesis Transferases/metabolism
Chemicals
Guanosine Guanine RNA, Transfer Transferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McKinnon R D
Wosnick M A
White B N
References (18)
18 references, click to expand
  1. Structure of the modified nucleoside Q isolated from Escherichia coli transfer ribonucleic acid. 7-(4,5-cis-Dihydroxy-1-cyclopenten-3-ylaminomethyl)-7-deazaguanosine.
    Biochemistry. 1975 Sep 23;14(19):4198-208 PMID: 1101947
  2. Distribution of the modified nucleoside Q and its derivatives in animal and plant transfer RNA's.
    Nucleic Acids Res. 1975 Oct;2(10):1931-9 PMID: 1187350
  3. A new method for the isolation of ribonucleic acids from mammalian tissues.
    Biochem J. 1956 Nov;64(3):405-8 PMID: 13373784
  4. Identification of the minor guanylated tRNA of rabbit reticulocytes.
    Nucleic Acids Res. 1976 Oct;3(10):2521-8 PMID: 186760
  5. Detection of nucleoside Q precursor in methyl-deficient E.coli tRNA.
    Nucleic Acids Res. 1977 Dec;4(12):4063-75 PMID: 341083
  6. Activity of a transfer RNA modifying enzyme during the development of Drosophila and its relationship to the su(s) locus.
    J Mol Biol. 1973 Mar 15;74(4):635-51 PMID: 4199662
  7. Guanylation of transfer RNA by rabbit reticulocytes.
    Biochim Biophys Acta. 1970 Jul 16;213(1):77-89 PMID: 4321274
  8. Chromatographic changes in specific tRNAs after reaction with cyanogen bromide and sodium periodate.
    Biochim Biophys Acta. 1974 Jul 11;353(3):283-91 PMID: 4368500
  9. Possible anticodon sequences of tRNA His , tRNA Asm , and tRNA Asp from Escherichia coli B. Universal presence of nucleoside Q in the first postion of the anticondons of these transfer ribonucleic acids.
    Biochemistry. 1972 Jan 18;11(2):301-8 PMID: 4550561
  10. Minor components in transfer RNA: their characterization, location, and function.
    Prog Nucleic Acid Res Mol Biol. 1972;12:49-85 PMID: 4557059
  11. Separation of transfer ribonucleic acids by reverse phase chromatography.
    J Biol Chem. 1965 Oct;240(10):3979-83 PMID: 5320645
  12. Biosynthesis of the pyrrolopyrimidine nucleoside antibiotic, toyocamycin. VII. Origin of the pyrrole carbons and the cyano carbon.
    J Biol Chem. 1970 Sep 10;245(17):4365-71 PMID: 5498424
  13. Improved separation of transfer RNA's on polychlorotrifuoroethylene-supported reversed-phase chromatography columns.
    Biochim Biophys Acta. 1971 Feb 11;228(3):770-4 PMID: 5572614
  14. Mechanism of suppression in Drosophila: a change in tyrosine transfer RNA.
    J Mol Biol. 1971 Apr 28;57(2):231-45 PMID: 5579621
  15. Specific replacement of Q base in the anticodon of tRNA by guanine catalyzed by a cell-free extract of rabbit reticulocytes.
    Nucleic Acids Res. 1976 Oct;3(10):2593-603 PMID: 792816
  16. Letter: The structure of Q* nucleoside isolated from rabbit liver transfer ribonucleic acid.
    J Am Chem Soc. 1976 Aug 4;98(16):5044-6 PMID: 950430
  17. Partial purification and properties of the reticulocyte guanylating enzyme.
    Biochim Biophys Acta. 1976 Sep 6;442(3):379-90 PMID: 963055
  18. Purification and nucleoside composition of a Q* - containing aspartic acid tRNA from Drosophila.
    Biochem Biophys Res Commun. 1978 Apr 28;81(4):1131-8 PMID: 96828
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.12.4865
Published
1978-12-00
Pages
4865-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342794
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.12.4865 PMC Full Text View journal details
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