The primary transcript of the ribosomal repeating unit in yeast.

Nucleic acids research ·Vol. 6 ·No. 1 ·1979-01-00 ·Pages 27-39

Klootwijk J, de Jonge P, Planta RJ

Abstract

Endgroup analysis of 37S ribosomal precursor RNA from Saccharomyces carlsbergensis has revealed that the major 5' endgroup is ppA-Up, with a molar yield of 0.8. This shows that most, if not all, 37S RNA molecules have preserved a transcriptional initiation sequence. Analysis of the 3' terminus of 37S RNA has shown the presence of a uridine rich oligonucleotide, tentatively identified as U6-8-A-NOH. This long stretch of uridines at the 3' end of 37S RNA may represent a transcriptional termination site. The two sets of data on the terminal sequences suggest that 37S ribosomal precursor RNA, if not already spliced, is a primary transcription product. Since the 3' terminus of 26S rRNA, U-U-U-G-UOH., appears to be clearly different from the 3' end of 37S RNA, we conclude that 37S ribosomal precursor RNA contains additional nucleotides 3'-distal to the 26S rRNA sequence.

MeSH Terms

Base Sequence Oligoribonucleotides/analysis RNA, Ribosomal/biosynthesis,isolation & purification Ribonuclease T1 Ribonucleases Ribosomes/metabolism Saccharomyces/metabolism Transcription, Genetic

Chemicals

Oligoribonucleotides RNA, Ribosomal Ribonucleases Ribonuclease T1

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Klootwijk J

de Jonge P

Planta R J

References (29)

29 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.1.27

Published

1979-01-00

Pages

27-39

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327671

Subset

External Links

PubMed source DOI: 10.1093/nar/6.1.27 PMC Full Text View journal details

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The primary transcript of the ribosomal repeating unit in yeast.

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