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PMID: 1064883 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

beta-Lipotropin as a prohormone for the morphinomimetic peptides endorphins and enkephalins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 6 ·1976-06-00 ·Pages 2156-9

Lazarus LH, Ling N, Guillemin R

Abstract

The hypophysial homomeric peptide beta-lipotropin (beta-LPH-[1-91]) has no morphinomomimetic activity in a bioassay (myenteric plexus-longitudinal muscle of the guinea pig's ileum) or binding assays with stereospecific opiate-receptors of rat brain synaptosome preparations. Incubating beta-LPH-[1-91] at neutral pH with the supernatant aqueous extracts of rat brain generates (fragments of beta-LPH with) morphinomimetic activity in the same assay systems. These results are related to the recently recognized structural relationships between beta-LPH, the newly isolated peptides met-enkephalin (beta-LPH-[61-65]) and alpha-endorphin (beta-LPH-[61-76]) and also to the biologically active fragments of analogs: beta-LPH-[61-64], beta-LPH-[61-65[-NH2, (Met(O)65)-BETA-LPH-[61-65], beta-LPH-[61-69], and beta-LPH-[61-69]. Enzymatic biogenesis of these morphinomimetic peptides would preclude localizing them as such in cellular or subcellular elements with currently available methodology.

MeSH Terms
Amino Acid Sequence Binding, Competitive Brain/metabolism Etorphine/metabolism Ileum/drug effects Morphine Derivatives/pharmacology Naloxone/pharmacology Nerve Tissue Proteins/biosynthesis,metabolism Oligopeptides/biosynthesis,metabolism Protein Precursors/metabolism Receptors, Drug Structure-Activity Relationship beta-Lipotropin/metabolism
Chemicals
Morphine Derivatives Nerve Tissue Proteins Oligopeptides Protein Precursors Receptors, Drug Naloxone Etorphine beta-Lipotropin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lazarus L H
Ling N
Guillemin R
References (21)
21 references, click to expand
  1. THE RENIN-ANGIOTENSIN SYSTEM.
    Pharmacol Rev. 1965 Jun;17:143-82 PMID: 14298579
  2. LIPOTROPIN, A NEW ACTIVE PEPTIDE FROM PITUITARY GLANDS.
    Nature. 1964 Feb 29;201:924 PMID: 14132790
  3. On a possible role of beta-melanocyte stimulating hormone (beta-MSH) in the central nervous system of the Mammalia: enzymatic inactivation in vitro of beta-MSH by brain tissue.
    Endocrinology. 1961 Jul;69:176-81 PMID: 13763329
  4. The origin of acetylcholine released from guinea-pig intestine and longitudinal muscle strips.
    J Physiol. 1968 Jan;194(1):13-33 PMID: 4295753
  5. Narcotic receptor sites in morphine-dependent rats.
    Nature. 1974 Mar 1;248(5443):61-3 PMID: 4131940
  6. Biological proprtie of ovine beta-lipotropic hormone.
    Endocrinology. 1968 May;82(5):898-904 PMID: 4171733
  7. Renin activity in dog brain: enzymological similarity to cathepsin D.
    Endocrinology. 1976 Jul;99(1):93-100 PMID: 181241
  8. Adrenocorticotrophic and melanocyte-stimulating peptides in the human pituitary.
    Biochem J. 1974 Jun;139(3):593-602 PMID: 4368382
  9. Angiotensin-forming enzyme in human brain.
    Neuropharmacology. 1975 Jan;14(1):75-80 PMID: 239362
  10. Angiotensin-converting enzyme in human brain.
    J Neurochem. 1975 Jul;25(1):83-5 PMID: 166145
  11. Immunohistochemical and histochemical studies of pituitary beta-lipotrophs.
    Anat Rec. 1973 Mar;175(3):529-37 PMID: 4349315
  12. Angiotensin-forming enzyme in brain tissue.
    Science. 1971 Jul 2;173(3991):64-5 PMID: 4325865
  13. Commentary: behaviourally active ACTH analogues.
    Biochem Pharmacol. 1975 Aug 15;24(16):1463-8 PMID: 172082
  14. Synthesis of a pentekontapeptide with high lipolytic activity corresponding to the carboxyl-terminal fifty amino acids of ovine beta-lipotropin.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4945-9 PMID: 4531028
  15. [Endorphins, hypothalamic and neurohypophysial peptides with morphinomimetic activity: isolation and molecular structure of alpha-endorphin].
    C R Acad Sci Hebd Seances Acad Sci D. 1976 Feb 23;282(8):783-5 PMID: 817843
  16. Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145-8 PMID: 1063395
  17. Opioid activity of a peptide, beta-lipotropin-(61-91), derived from beta-lipotropin.
    Proc Natl Acad Sci U S A. 1976 Jun;73(6):1821-3 PMID: 1064855
  18. Identification of two related pentapeptides from the brain with potent opiate agonist activity.
    Nature. 1975 Dec 18;258(5536):577-80 PMID: 1207728
  19. A peptide-like substance from pituitary that acts like morphine. 2. Purification and properties.
    Life Sci. 1975 Jun 15;16(12):1777-82 PMID: 1152601
  20. Inactivation of somatostatin (GH-RIH) and its an analogs by crude and partially purified rat brain extracts.
    FEBS Lett. 1975 Jul 15;55(1):220-4 PMID: 1140419
  21. The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').
    Biochem J. 1964 Feb;90(2):293-303 PMID: 5834239
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.6.2156
Published
1976-06-00
Pages
2156-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430469
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.6.2156 PMC Full Text View journal details
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