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PMID: 10655501 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Recent, extensive, and preferential insertion of members of the miniature inverted-repeat transposable element family Heartbreaker into genic regions of maize.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 3 ·2000-02-01 ·Pages 1160-5

Zhang Q, Arbuckle J, Wessler SR

Abstract

A 314-bp DNA element called Heartbreaker-hm1 (Hbr-hm1) was previously identified in the 3' untranslated region of a mutant allele of the maize disease resistance gene HM1. This element has structural features of miniature inverted-repeat transposable elements (MITEs) and is a member of a large family of approximately 4,000 copies in the maize genome. Unlike previously described MITEs, most members of the Hbr family display over 90% sequence identity. This, coupled with the insertion of an Hbr element into an allele of the HM1 gene, suggested that this family might have spread recently throughout the genome. Consistent with this view is the finding that Hbr insertion sites are remarkably polymorphic. Ten of ten loci containing Hbr elements were found to be polymorphic for the presence or absence of Hbr among a collection of maize inbred lines and teosinte strains. Despite the fact that over 80% of the maize genome contain moderate to highly repetitive DNA, we find that randomly chosen Hbr elements are predominantly in single or low copy regions. Furthermore, when used to query both the public and private databases of plant genes, over 50% of the sequences flanking these Hbr elements resulted in significant "hits." Taken together, these data indicate that the presence or absence of Hbr elements is a significant contributory factor to the high level of polymorphism associated with maize genic regions.

MeSH Terms

Base Sequence DNA Transposable Elements Genes, Plant Genetic Variation Molecular Sequence Data Zea mays/genetics

Chemicals

DNA Transposable Elements

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Zhang Q

Departments of Botany and Genetics, University of Georgia, Athens, GA 30602, USA.

Arbuckle J

Wessler S R

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.97.3.1160

Published

2000-02-01

Pages

1160-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC15555

Subset

Databases

GENBANK

AF205891, AF205892, AF205893, AF205894, AF205895, AF205896, AF205897, AF205898, AF205899, AF205900, AF205901, AF205902, AF205903, AF205904, AF205905, AF205906, AF205907, AF205908, AF205909, AF205910, AF205911, AF205912, AF205913, AF205914, AF205915, AF205916, AF205917, AF205918, AF205919, AF205920

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PubMed source DOI: 10.1073/pnas.97.3.1160 PMC Full Text View journal details

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Recent, extensive, and preferential insertion of members of the miniature inverted-repeat transposable element family Heartbreaker into genic regions of maize.

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