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PMID: 1065886 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Host restriction of Friend leukemia virus: synthesis and integration of the provirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2356-60

Sveda MM, Soeiro R

Abstract

Host restriction of exogenous infection by murine leukemia viruses is controlled in vitro predominantly by the murine Fv-1 locus. The mechanism of this host restriction was investigated by comparing the early events in the replication of N-tropic versus B-tropic Friend leukemia virus in NIH 3T3 cells. These cells, which are Fv-1nn in type, are permissive for the N-tropic strain, but nonpermissive for the B-tropic strain, which replicates permissively in Balb/c cells. We have studied the synthesis, intracellular location, and molecular form of virus-specific DNA early in replication by means of molecular hybridization with a virus-specific DNA probe. Our results suggest that in the permissive infection viral DNA rapidly becomes integrated with cellular DNA. However, in the nonpermissive infection, although almost equal amounts of both positive and negative strand viral DNA are synthesized, integration of the provirus does not occur.

MeSH Terms
Cell Line DNA/metabolism DNA, Circular/analysis DNA, Viral/analysis,biosynthesis,metabolism Friend murine leukemia virus/metabolism Genes Nucleic Acid Conformation Virus Replication
Chemicals
DNA, Circular DNA, Viral DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sveda M M
Soeiro R
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2356
Published
1976-07-00
Pages
2356-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430564
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2356 PMC Full Text View journal details
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