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PMID: 1065893 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Enhancement of postreplication repair in Chinese hamster cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2396-400

D'Ambrosio SM, Setlow RB

Abstract

Alkaline sedimentation profiles of pulse-labeled DNA from Chinese hamster cells showed that DNA from cells treated with N-acetoxy-acetylaminofluorene or ultraviolet radiation was made in segments smaller than those from untreated cells. Cells treated with a small dose (2.5 muM) of N-acetoxy-acetylaminofluorene or (2.5 J-m-2) 254-nm radiation, several hours before a larger dose (7-10 muM) of N-acetoxy-acetylaminofluorene or 5.0 J.m-2 of 254 nm radiation, also synthesized small DNA after the second dose. However, the rate at which this small DNA was joined together into parental size was appreciably greater than in absence of the small dose. This enhancement of postreplication repair (as a result of the initial small dose) was not observed when cells were incubated with cycloheximide between the two treatments. The results suggest that N-acetoxy-acetylaminofluorene and ultraviolet-damaged DNA from Chinese hamster cells are repaired by similar postreplicative mechanisms that require de novo protein synthesis for enhancement.

MeSH Terms
Acetoxyacetylaminofluorene/pharmacology Cell Line Cycloheximide/pharmacology DNA/biosynthesis DNA Repair/drug effects,radiation effects DNA Replication Dose-Response Relationship, Drug Dose-Response Relationship, Radiation Molecular Weight Radiation Effects Ultraviolet Rays
Chemicals
Acetoxyacetylaminofluorene DNA Cycloheximide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
D'Ambrosio S M
Setlow R B
References (26)
26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2396
Published
1976-07-00
Pages
2396-400
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430582
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2396 PMC Full Text View journal details
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