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PMID: 10675334 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription factor Sp3 is essential for post-natal survival and late tooth development.

The EMBO journal ·Vol. 19 ·No. 4 ·2000-02-15 ·Pages 655-61

Bouwman P, Göllner H, Elsässer HP, Eckhoff G, Karis A, Grosveld F, Philipsen S, Suske G

Abstract

Sp3 is a ubiquitously expressed transcription factor closely related to Sp1 (specificity protein 1). We have disrupted the mouse Sp3 gene by homologous recombination. Sp3-deficient embryos are growth retarded and invariably die at birth of respiratory failure. The cause for the observed breathing defect remains obscure since only minor morphological alterations were observed in the lung, and surfactant protein expression is indistinguishable from that in wild-type mice. Histological examinations of individual organs in Sp3(-/-) mice show a pronounced defect in late tooth formation. In Sp3 null mice, the dentin/enamel layer of the developing teeth is impaired due to the lack of ameloblast-specific gene products. Comparison of the Sp1 and Sp3 knockout phenotype shows that Sp1 and Sp3 have distinct functions in vivo, but also suggests a degree of functional redundancy.

MeSH Terms
Ameloblasts/metabolism Animals Animals, Newborn Base Sequence DNA Primers/genetics DNA-Binding Proteins/deficiency,genetics,physiology Female Gene Expression Lung/metabolism,pathology Male Mice Mice, Inbred C57BL Mice, Knockout Odontogenesis/genetics,physiology Phenotype Pregnancy Respiratory Insufficiency/genetics,metabolism,pathology Sp1 Transcription Factor/deficiency,genetics,physiology Sp3 Transcription Factor Tooth/growth & development,metabolism,pathology Transcription Factors/deficiency,genetics,physiology
Chemicals
DNA Primers DNA-Binding Proteins Sp1 Transcription Factor Sp3 protein, mouse Transcription Factors Sp3 Transcription Factor
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Bouwman P
Institut für Molekularbiologie und Tumorforschung, Emil-Mannkopff-Strasse 2.
Göllner H
Elsässer H P
Eckhoff G
Karis A
Grosveld F
Philipsen S
Suske G
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35 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.4.655
Published
2000-02-15
Pages
655-61
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305603
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.4.655 PMC Full Text View journal details
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