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PMID: 1454515 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes.

Nucleic acids research ·Vol. 20 ·No. 21 ·1992-11-11 ·Pages 5519-25

Hagen G, Müller S, Beato M, Suske G

Abstract

Previous analyses of the uteroglobin gene promoter revealed a GT1 box which is also found in the SV40 enhancer. The GT1 element in the context of the uteroglobin promoter is active in Ishikawa cells, a human endometrial cell line, but not in HeLa cells. Here we report the cloning by recognition site screening of two factors (SPR-1 and SPR-2) which bind to this GT1 motif. SPR-1 and SPR-2 are homologues of the transcription factor Sp1. All three proteins are closely related members of a gene family encoding proteins with very similar structural features. Like Sp1, SPR-1 and SPR-2 contain glutamine and serine/threonine rich amino acid stretches. Most significantly, the DNA binding domains of all three proteins are highly conserved and they recognize GT as well as GC boxes identically. SPR-2 mRNA is expressed ubiquitously, whereas SPR-1 transcripts are abundant in the brain but barely detectable in other organs. The possible function of these factors for the activity of the uteroglobin promoter is discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding Sites Blotting, Northern Brain/metabolism Cell Line Cloning, Molecular/methods DNA/metabolism DNA-Binding Proteins/genetics,metabolism Humans Mice Molecular Sequence Data Multigene Family Organ Specificity Polymerase Chain Reaction Promoter Regions, Genetic Rabbits Sequence Homology, Amino Acid Sp1 Transcription Factor/genetics,metabolism Sp3 Transcription Factor Sp4 Transcription Factor Transcription, Genetic Uteroglobin/genetics
Chemicals
DNA-Binding Proteins SP4 protein, human Sp1 Transcription Factor Sp4 Transcription Factor Sp4 protein, mouse Sp3 Transcription Factor DNA Uteroglobin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hagen G
Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Germany.
Müller S
Beato M
Suske G
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.21.5519
Published
1992-11-11
Pages
5519-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334381
Subset
IM
Databases
GENBANK
X68560, X68561
External Links
PubMed source DOI: 10.1093/nar/20.21.5519 PMC Full Text View journal details
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