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PMID: 10691737 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Yeast heterochromatin is a dynamic structure that requires silencers continuously.

Genes & development ·Vol. 14 ·No. 4 ·2000-02-15 ·Pages 452-63

Cheng TH, Gartenberg MR

Abstract

Transcriptional silencing of the HM loci in yeast requires cis-acting elements, termed silencers, that function during S-phase passage to establish the silent state. To study the role of the regulatory elements in maintenance of repression, site-specific recombination was used to uncouple preassembled silent chromatin fragments from silencers. DNA rings excised from HMR were initially silent but ultimately reactivated, even in G(1)- or G(2)/M-arrested cells. In contrast, DNA rings bearing HML-derived sequence were stably repressed due to the presence of a protosilencing element. These data show that silencers (or protosilencers) are required continuously for maintenance of silent chromatin. Reactivation of unstably repressed rings was blocked by overexpression of silencing proteins Sir3p and Sir4p, and chromatin immunoprecipitation studies showed that overexpressed Sir3p was incorporated into silent chromatin. Importantly, the protein was incorporated even when expressed outside of S phase, during G(1) arrest. That silencing factors can associate with and stabilize preassembled silent chromatin in non-S-phase cells demonstrates that heterochromatin in yeast is dynamic.

MeSH Terms
Chromosomes, Fungal/genetics,ultrastructure DNA, Circular/genetics DNA, Fungal/genetics Fungal Proteins/genetics,physiology G1 Phase Gene Silencing Genes, Fungal Heterochromatin/genetics Mating Factor Peptides/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics Silent Information Regulator Proteins, Saccharomyces cerevisiae Trans-Activators/physiology
Chemicals
DNA, Circular DNA, Fungal Fungal Proteins Heterochromatin Peptides SIR3 protein, S cerevisiae SIR4 protein, S cerevisiae Silent Information Regulator Proteins, Saccharomyces cerevisiae Trans-Activators Mating Factor
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cheng T H
Department of Pharmacology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, New Jersey 08854 USA.
Gartenberg M R
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
Published
2000-02-15
Pages
452-63
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316382
Subset
IM
Grants
NIGMS NIH HHS · R01 GM051402 · United States
NIGMS NIH HHS · GM51402 · United States
External Links
PubMed source PMC Full Text View journal details
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