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PMID: 172901 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Conformational fluctuations of DNA helix.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 11 ·1975-11-00 ·Pages 4275-9

Depew DE, Wang JC

Abstract

Treatment of a homogeneous sample of circular DNA molecules that contain at least one single-strand scission per molecule with ligase yields a population of covalently closed circles heterogeneous in the topological winding number alpha. Under proper conditions, the product molecules with different values of alpha can all be resolved by gel electrophoresis. The distributions in alpha for several DNAs, covalently closed at different temperatures, have been determined and the results have been interpreted in terms of thermal fluctuations of the DNA helix.

MeSH Terms

Bacteriophages Coliphages DNA, Bacterial DNA, Circular/isolation & purification DNA, Viral Nucleic Acid Conformation Simian virus 40 Temperature

Chemicals

DNA, Bacterial DNA, Circular DNA, Viral

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Depew D E

Wang J C

References (15)

15 references, click to expand

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J Mol Biol. 1969 Jul 14;43(1):25-39 PMID: 4897794
Effect of DNA length on the free energy of binding of an unwinding ligand to a supercoiled DNA.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.11.4275

Published

1975-11-00

Pages

4275-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC388703

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.11.4275 PMC Full Text View journal details

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