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PMID: 10692363 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

EFG1 null mutants of Candida albicans switch but cannot express the complete phenotype of white-phase budding cells.

Journal of bacteriology ·Vol. 182 ·No. 6 ·2000-03-00 ·Pages 1580-91

Srikantha T, Tsai LK, Daniels K, Soll DR

Abstract

The Candida albicans gene EFG1 encodes a putative trans-acting factor. In strain WO-1, which undergoes the white-opaque transition, EFG1 is transcribed as a 3.2-kb mRNA in white-phase cells and a less-abundant 2.2-kb mRNA in opaque-phase cells. cDNA sequencing and 5' rapid amplification of cDNA ends analysis demonstrate that the major difference in molecular mass of the two transcripts is due to different transcription start sites. EFG1 null mutants form opaque-phase colonies and express the opaque-phase cell phenotype at 25 degrees C. When shifted from 25 to 42 degrees C, mutant opaque-phase cells undergo phenotypic commitment to the white phase, which includes deactivation of the opaque-phase-specific gene OP4 and activation of the white-phase-specific gene WH11, as do wild-type opaque-phase cells. After the commitment event, EFG1 null mutant cells form daughter cells which have the smooth (pimpleless) surface of white-phase cells but the elongate morphology of opaque-phase cells. Taken together, these results demonstrate that EFG1 expression is not essential for the switch event per se, but is essential for a subset of phenotypic characteristics necessary for the full expression of the phenotype of white-phase cells. These results demonstrate that EFG1 is not the site of the switch event, but is, rather, downstream of the switch event.

MeSH Terms
5' Untranslated Regions/genetics Base Sequence Blotting, Northern Blotting, Southern Candida albicans/genetics,growth & development,metabolism,ultrastructure Colony Count, Microbial DNA, Complementary/genetics DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Deletion Gene Expression Regulation, Fungal Microscopy, Electron, Scanning Molecular Sequence Data Mutation Phenotype Promoter Regions, Genetic Sequence Analysis, DNA Transcription Factors Transcription, Genetic
Chemicals
5' Untranslated Regions DNA, Complementary DNA-Binding Proteins EFG1 protein, Candida albicans Fungal Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Srikantha T
Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA.
Tsai L K
Daniels K
Soll D R
References (48)
48 references, click to expand
  1. Regulation of phase-specific genes in the more general switching system of Candida albicans strain 3153A.
    J Med Vet Mycol. 1994;32(4):287-94 PMID: 7983573
  2. Differences in adhesion of Candida albicans 3153A cells exhibiting switch phenotypes to buccal epithelium and stratum corneum.
    Infect Immun. 1994 Apr;62(4):1328-35 PMID: 8132340
  3. Increased phenotypic switching in strains of Candida albicans associated with invasive infections.
    J Clin Microbiol. 1994 Nov;32(11):2869-70 PMID: 7852592
  4. The frequency of integrative transformation at phase-specific genes of Candida albicans correlates with their transcriptional state.
    Mol Gen Genet. 1995 Feb 6;246(3):342-52 PMID: 7854319
  5. Functional analysis of the promoter of the phase-specific WH11 gene of Candida albicans.
    Mol Cell Biol. 1995 Mar;15(3):1797-805 PMID: 7862169
  6. SOK2 may regulate cyclic AMP-dependent protein kinase-stimulated growth and pseudohyphal development by repressing transcription.
    Mol Cell Biol. 1995 Dec;15(12):6854-63 PMID: 8524252
  7. The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans.
    J Bacteriol. 1996 Jan;178(1):121-9 PMID: 8550405
  8. Asm-1+, a Neurospora crassa gene related to transcriptional regulators of fungal development.
    Genetics. 1996 Nov;144(3):991-1003 PMID: 8913744
  9. Gene regulation during high-frequency switching in Candida albicans.
    Microbiology. 1997 Feb;143 ( Pt 2):279-88 PMID: 9043104
  10. Efg1p, an essential regulator of morphogenesis of the human pathogen Candida albicans, is a member of a conserved class of bHLH proteins regulating morphogenetic processes in fungi.
    EMBO J. 1997 Apr 15;16(8):1982-91 PMID: 9155024
  11. The WH11 gene of Candida albicans is regulated in two distinct developmental programs through the same transcription activation sequences.
    J Bacteriol. 1997 Jun;179(12):3837-44 PMID: 9190797
  12. Nonfilamentous C. albicans mutants are avirulent.
    Cell. 1997 Sep 5;90(5):939-49 PMID: 9298905
  13. Aspergillus asexual reproduction and sexual reproduction are differentially affected by transcriptional and translational mechanisms regulating stunted gene expression.
    Mol Cell Biol. 1997 Oct;17(10):6191-201 PMID: 9315680
  14. Misexpression of the white-phase-specific gene WH11 in the opaque phase of Candida albicans affects switching and virulence.
    Infect Immun. 1997 Nov;65(11):4468-75 PMID: 9353021
  15. The Candida albicans CDR3 gene codes for an opaque-phase ABC transporter.
    J Bacteriol. 1997 Dec;179(23):7210-8 PMID: 9393682
  16. Switch of phenotype as an escape mechanism of the intruder.
    Mycoses. 1997;40 Suppl 2:9-12 PMID: 9476497
  17. The two-component hybrid kinase regulator CaNIK1 of Candida albicans.
    Microbiology. 1998 Oct;144 ( Pt 10):2715-29 PMID: 9802013
  18. A MADS box protein consensus binding site is necessary and sufficient for activation of the opaque-phase-specific gene OP4 of Candida albicans.
    J Bacteriol. 1998 Dec;180(24):6607-16 PMID: 9852005
  19. Control of white-opaque phenotypic switching in Candida albicans by the Efg1p morphogenetic regulator.
    Infect Immun. 1999 Sep;67(9):4655-60 PMID: 10456912
  20. Phenotypic switching in Candida glabrata involves phase-specific regulation of the metallothionein gene MT-II and the newly discovered hemolysin gene HLP.
    Infect Immun. 2000 Feb;68(2):884-95 PMID: 10639459
  21. Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans: evidence for zinc-resistant and -sensitive pathways for mycelium formation.
    Infect Immun. 1979 Oct;26(1):348-54 PMID: 387610
  22. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  23. High-frequency switching of colony morphology in Candida albicans.
    Science. 1985 Nov 8;230(4726):666-9 PMID: 3901258
  24. Integrative transformation of Candida albicans, using a cloned Candida ADE2 gene.
    Mol Cell Biol. 1986 Jan;6(1):142-9 PMID: 3023819
  25. "White-opaque transition": a second high-frequency switching system in Candida albicans.
    J Bacteriol. 1987 Jan;169(1):189-97 PMID: 3539914
  26. High-frequency switching in Candida strains isolated from vaginitis patients.
    J Clin Microbiol. 1987 Sep;25(9):1611-22 PMID: 2821060
  27. Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements.
    Cell. 1987 Dec 4;51(5):721-32 PMID: 3315231
  28. Unique phenotype of opaque cells in the white-opaque transition of Candida albicans.
    J Bacteriol. 1987 Dec;169(12):5579-88 PMID: 3316187
  29. Opaque-white phenotype transition: a programmed morphological transition in Candida albicans.
    J Bacteriol. 1988 Feb;170(2):895-9 PMID: 2828333
  30. A yeast activity can substitute for the HeLa cell TATA box factor.
    Nature. 1988 Jul 7;334(6177):77-80 PMID: 3290688
  31. Multiple Candida strains in the course of a single systemic infection.
    J Clin Microbiol. 1988 Aug;26(8):1448-59 PMID: 3049653
  32. Variation in adhesion and cell surface hydrophobicity in Candida albicans white and opaque phenotypes.
    Mycopathologia. 1988 Jun;102(3):149-56 PMID: 3050525
  33. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
    Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 PMID: 2461560
  34. A highly conserved sequence in yeast heat shock gene promoters.
    Nucleic Acids Res. 1988 Dec 23;16(24):11845 PMID: 3062587
  35. Hypha formation in the white-opaque transition of Candida albicans.
    Infect Immun. 1989 Feb;57(2):458-67 PMID: 2643570
  36. Ultrastructure and antigenicity of the unique cell wall pimple of the Candida opaque phenotype.
    J Bacteriol. 1990 Jan;172(1):224-35 PMID: 2403540
  37. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
    Curr Genet. 1989 Dec;16(5-6):339-46 PMID: 2692852
  38. Sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast Candida albicans.
    J Bacteriol. 1990 Apr;172(4):2036-45 PMID: 2180914
  39. High-frequency switching in Candida albicans.
    Clin Microbiol Rev. 1992 Apr;5(2):183-203 PMID: 1576587
  40. Transcription of the gene for a pepsinogen, PEP1, is regulated by white-opaque switching in Candida albicans.
    Mol Cell Biol. 1992 Jul;12(7):2997-3005 PMID: 1620110
  41. StuA is required for cell pattern formation in Aspergillus.
    Genes Dev. 1992 Sep;6(9):1770-82 PMID: 1516832
  42. Coordinate regulation of two opaque-phase-specific genes during white-opaque switching in Candida albicans.
    Infect Immun. 1993 May;61(5):1823-8 PMID: 8478072
  43. Isogenic strain construction and gene mapping in Candida albicans.
    Genetics. 1993 Jul;134(3):717-28 PMID: 8349105
  44. A white-specific gene in the white-opaque switching system of Candida albicans.
    Gene. 1993 Sep 6;131(1):53-60 PMID: 7916716
  45. Three distinct secreted aspartyl proteinases in Candida albicans.
    J Bacteriol. 1993 Oct;175(19):6126-33 PMID: 8407785
  46. Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity.
    J Clin Microbiol. 1993 Dec;31(12):3190-9 PMID: 8308110
  47. Induction of pseudohyphal growth by overexpression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development.
    Mol Cell Biol. 1994 Mar;14(3):2100-12 PMID: 8114741
  48. Expression of seven members of the gene family encoding secretory aspartyl proteinases in Candida albicans.
    Mol Microbiol. 1994 Oct;14(1):87-99 PMID: 7830564
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.6.1580-1591.2000
Published
2000-03-00
Pages
1580-91
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94455
Subset
IM
Grants
NIAID NIH HHS · AI2392 · United States
NIDCR NIH HHS · DE1058 · United States
External Links
PubMed source DOI: 10.1128/JB.182.6.1580-1591.2000 PMC Full Text View journal details
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