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PMID: 9353021 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Misexpression of the white-phase-specific gene WH11 in the opaque phase of Candida albicans affects switching and virulence.

Infection and immunity ·Vol. 65 ·No. 11 ·1997-11-00 ·Pages 4468-75

Kvaal CA, Srikantha T, Soll DR

Abstract

Candida albicans WO-1 switches between a white- and an opaque-colony-forming phenotype. The gene WH11 is expressed differentially in the white phase. The WH11 open reading frame was inserted downstream of the promoter of the opaque-phase-specific gene OP4 in the transforming vector pCWOP16, and resulting transformants were demonstrated to misexpress WH11 in the opaque phase. Misexpression had no effect on the ability to switch from the white to the opaque or the opaque to the white phase, and it had no effect on the genesis of the unique opaque-phase cellular phenotype, even though the Wh11 protein was distributed throughout the cytoplasm in a manner similar to that observed for the endogenous gene product in the white phase. Misexpression did, however, increase the frequency of the opaque-to-white transition 330-fold and markedly increased the virulence of cells in the opaque phase in a mouse tail injection model.

MeSH Terms
Animals Candida albicans/genetics,pathogenicity Female Fungal Proteins/biosynthesis Gene Expression Regulation, Fungal Genes, Fungal Mice Transformation, Genetic Virulence
Chemicals
Fungal Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kvaal C A
Department of Biological Sciences, The University of Iowa, Iowa City 52242, USA.
Srikantha T
Soll D R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.65.11.4468-4475.1997
Published
1997-11-00
Pages
4468-75
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC175642
Subset
IM
Grants
NIAID NIH HHS · AI39735 · United States
NIDCR NIH HHS · DE10758 · United States
External Links
PubMed source DOI: 10.1128/iai.65.11.4468-4475.1997 PMC Full Text View journal details
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