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PMID: 7565776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Saccharomyces cerevisiae HSP12 gene is activated by the high-osmolarity glycerol pathway and negatively regulated by protein kinase A.

Molecular and cellular biology ·Vol. 15 ·No. 11 ·1995-11-00 ·Pages 6232-45

Varela JC, Praekelt UM, Meacock PA, Planta RJ, Mager WH

Abstract

The HSP12 gene encodes one of the two major small heat shock proteins of Saccharomyces cerevisiae. Hsp12 accumulates massively in yeast cells exposed to heat shock, osmostress, oxidative stress, and high concentrations of alcohol as well as in early-stationary-phase cells. We have cloned an extended 5'-flanking region of the HSP12 gene in order to identify cis-acting elements involved in regulation of this highly expressed stress gene. A detailed analysis of the HSP12 promoter region revealed that five repeats of the stress-responsive CCCCT motif (stress-responsive element [STRE]) are essential to confer wild-type induced levels on a reporter gene upon osmostress, heat shock, and entry into stationary phase. Disruption of the HOG1 and PBS2 genes leads to a dramatic decrease of the HSP12 inducibility in osmostressed cells, whereas overproduction of Hog1 produces a fivefold increase in wild-type induced levels upon a shift to a high salt concentration. On the other hand, mutations resulting in high protein kinase A (PKA) activity reduce or abolish the accumulation of the HSP12 mRNA in stressed cells. Conversely, mutants containing defective PKA catalytic subunits exhibit high basal levels of HSP12 mRNA. Taken together, these results suggest that HSP12 is a target of the high-osmolarity glycerol (HOG) response pathway under negative control of the Ras-PKA pathway. Furthermore, they confirm earlier observations that STRE-like sequences are responsive to a broad range of stresses and that the HOG and Ras-PKA pathways have antagonistic effects upon CCCCT-driven transcription.

MeSH Terms
Consensus Sequence Cyclic AMP-Dependent Protein Kinases/physiology DNA Primers/chemistry DNA-Binding Proteins/metabolism,physiology Gene Expression Regulation, Fungal Genes, Fungal Heat-Shock Proteins/genetics Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions, Genetic RNA, Fungal/genetics RNA, Messenger/genetics Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Signal Transduction Structure-Activity Relationship Transcription Factors/metabolism,physiology Water-Electrolyte Balance
Chemicals
CAD1 protein, S cerevisiae DNA Primers DNA-Binding Proteins Heat-Shock Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Proteins Transcription Factors YAP1 protein, S cerevisiae Cyclic AMP-Dependent Protein Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Varela J C
Department of Biochemistry and Molecular Biology, Instituut voor Moleculair Biologische Wetenschappen, BioCentrum Amsterdam, Vrije Universiteit, The Netherlands.
Praekelt U M
Meacock P A
Planta R J
Mager W H
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.11.6232
Published
1995-11-00
Pages
6232-45
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230875
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.15.11.6232 PMC Full Text View journal details
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