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PMID: 10692383 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

AcrD of Escherichia coli is an aminoglycoside efflux pump.

Journal of bacteriology ·Vol. 182 ·No. 6 ·2000-03-00 ·Pages 1754-6

Rosenberg EY, Ma D, Nikaido H

Abstract

AcrD, a transporter belonging to the resistance-nodulation-division family, was shown to participate in the efflux of aminoglycosides. Deletion of the acrD gene decreased the MICs of amikacin, gentamicin, neomycin, kanamycin, and tobramycin by a factor of two to eight, and DeltaacrD cells accumulated higher levels of [(3)H]dihydrostreptomycin and [(3)H]gentamicin than did the parent strain.

MeSH Terms
Aminoglycosides Anti-Bacterial Agents/metabolism,pharmacology Carrier Proteins/genetics,metabolism Drug Resistance, Microbial/genetics Escherichia coli/drug effects,growth & development,metabolism Escherichia coli Proteins/genetics,metabolism Gene Deletion Membrane Transport Proteins/genetics,metabolism Microbial Sensitivity Tests
Chemicals
AcrD protein, E coli Aminoglycosides Anti-Bacterial Agents Carrier Proteins Escherichia coli Proteins Membrane Transport Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rosenberg E Y
Departments of Molecular and Cell Biology, University of California, Berkeley, California 94720-3206, USA.
Ma D
Nikaido H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.182.6.1754-1756.2000
Published
2000-03-00
Pages
1754-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC94475
Subset
IM
Grants
NIAID NIH HHS · R01 AI009644 · United States
NIAID NIH HHS · R37 AI009644 · United States
NIAID NIH HHS · AI-09644 · United States
External Links
PubMed source DOI: 10.1128/JB.182.6.1754-1756.2000 PMC Full Text View journal details
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