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PMID: 9765586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Relationship between spontaneous aminoglycoside resistance in Escherichia coli and a decrease in oligopeptide binding protein.

Journal of bacteriology ·Vol. 180 ·No. 20 ·1998-10-00 ·Pages 5484-8

Kashiwagi K, Tsuhako MH, Sakata K, Saisho T, Igarashi A, da Costa SO, Igarashi K

Abstract

Changes in the amount of oligopeptide binding protein (OppA) in spontaneous kanamycin-resistant mutants of Escherichia coli were investigated. Among 20 colonies obtained from 10(8) cells cultured in the presence of 20 microgram of kanamycin/ml, 1 colony had no detectable OppA and 7 colonies were mutants with reduced amounts of OppA. Sensitivity of wild-type cells to kanamycin increased slightly by transformation of the oppA gene, but the sensitivity of the mutants increased greatly by the transformation. A mutant with no OppA was found to be a nonsense mutant of the oppA gene at amino acid position 166. In a mutant having a reduced level of OppA, the reduction was due to the decrease in OppA synthesis at the translational level. These mutants were also resistant to other aminoglycoside antibiotics, including streptomycin, neomycin, and isepamicin. Isepamicin uptake activities decreased greatly in these two kinds of mutants. The results support the proposition that aminoglycoside antibiotics are transported into cells by the oligopeptide transport system, and that transport is an important factor for spontaneous resistance to aminoglycoside antibiotics.

MeSH Terms
Amino Acids/analysis Anti-Bacterial Agents/metabolism,pharmacology Bacterial Proteins Biological Transport/genetics Carrier Proteins/biosynthesis,genetics Down-Regulation Escherichia coli/drug effects,genetics Escherichia coli Proteins Gentamicins/metabolism Kanamycin/metabolism,pharmacology Kanamycin Resistance/genetics Lipoproteins/biosynthesis,genetics Mutagenesis Peptides/metabolism Polyamines/analysis
Chemicals
Amino Acids Anti-Bacterial Agents Bacterial Proteins Carrier Proteins Escherichia coli Proteins Gentamicins Lipoproteins OppA protein, E coli Peptides Polyamines oligopeptide-binding protein, bacteria polyphenylalanine Kanamycin isepamicin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Kashiwagi K
Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan.
Tsuhako M H
Sakata K
Saisho T
Igarashi A
da Costa S O
Igarashi K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.20.5484-5488.1998
Published
1998-10-00
Pages
5484-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107603
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.20.5484-5488.1998 PMC Full Text View journal details
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