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PMID: 6173015 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Role of the membrane potential in bacterial resistance to aminoglycoside antibiotics.

Antimicrobial agents and chemotherapy ·Vol. 20 ·No. 6 ·1981-12-00 ·Pages 803-8

Damper PD, Epstein W

Abstract

The electrical potential difference (delta psi) across the membrane of Escherichia coli was measured by the distribution of lipid-soluble cations and correlated with resistance to dihydrostreptomycin, where resistance is presumed due to reduced uptake of the drug. A good correlation between the two measured parameters was found under all conditions tested, which included effects of several mutations, inhibitors, changes in pH, and osmolarity. The most dramatic changes were seen when pH was varied; in wild-type strains resistance increased more than 100-fold, and delta psi fell by 70 mV when pH was reduced from 8.5 to 5.5. These results were interpreted as support for a model in which the uptake of the polycationic aminoglycosides is electrogenic and therefore driven by delta psi. The factor common to mutations and conditions which increase resistance was a reduction in delta psi. A simple model was developed which relates the minimal inhibitory concentration to the rate of aminoglycoside uptake and the rate of growth.

MeSH Terms
2,4-Dinitrophenol Aminoglycosides/pharmacology Anti-Bacterial Agents/pharmacology Cell Membrane/physiology Dihydrostreptomycin Sulfate/pharmacology Dinitrophenols/pharmacology Drug Resistance, Microbial Escherichia coli/drug effects,ultrastructure Membrane Potentials Microbial Sensitivity Tests
Chemicals
Aminoglycosides Anti-Bacterial Agents Dinitrophenols 2,4-Dinitrophenol Dihydrostreptomycin Sulfate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Damper P D
Epstein W
References (26)
26 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.20.6.803
Published
1981-12-00
Pages
803-8
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC181802
Subset
IM
Grants
NIGMS NIH HHS · GM22323 · United States
External Links
PubMed source DOI: 10.1128/AAC.20.6.803 PMC Full Text View journal details
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