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PMID: 1069277 Published · ppublish English Journal Article

DNA methylation in adenovirus, adenovirus-transformed cells, and host cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 3923-7

Gunthert U, Schweiger M, Stupp M, Doerfler W

Abstract

DNAs of adenovirus type 2 and type 12 contain low amounts of methylated bases (0.01 and 0.02% N6-methyl-adenine per adenine, if any, and 0.04 and 0.06% 5-methylcytosine per cytosine for type 2 and type 12, respectively), whereas the DNA of the mammalian host cells contains much more 5-methylcytosine (3.57% for human KB cells). The DNA of hamster cells transformed by adenovirus type 12 contains 3.11 and 3.14% 5-methycytosine (HA12/7 and T627 cells, respectively), whereas the DNA from untransformed hamster cells (BHK21 cells) contains 2.22% 5-methylcytosine. In the DNA of human and hamster cells, little, if any, N6-methyladenine was detected. Methylation of DNA was determined by a sensitive method based on two consecutive steps of two-dimensional thin-layer chromatography of the radioactively labeled DNA bases. By this procedure the detection limits of 5-methylcytosine and N6-methyladenine could be lowered to 0.01% per main base.

MeSH Terms
Adenoviridae/metabolism Cell Line Cell Transformation, Neoplastic Chemical Phenomena Chemistry Chromatography, Thin Layer DNA/analysis,metabolism DNA (Cytosine-5-)-Methyltransferases/metabolism DNA, Viral/metabolism Methyltransferases/metabolism
Chemicals
DNA, Viral DNA Methyltransferases DNA (Cytosine-5-)-Methyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gunthert U
Schweiger M
Stupp M
Doerfler W
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.11.3923
Published
1976-11-00
Pages
3923-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431267
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.11.3923 PMC Full Text View journal details
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