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PMID: 5085550 Published · ppublish English Journal Article

Biomethylation of deoxyribonucleic acid in cultured human tumour cells (HeLa). Methylated bases other than 5-methylcytosine not detected.

The Biochemical journal ·Vol. 128 ·No. 1 ·1972-06-00 ·Pages 133-8

Lawley PD, Crathorn AR, Shah SA, Smith BA

Abstract

1. Incorporation of methyl groups from [methyl-(14)C]methionine into DNA of dividing HeLa cells was investigated, essentially by the procedures of Culp, et al. (1970). 2. Contrary to the report of the latter, but in agreement with other work on biomethylation of mammalian DNA, 5-methylcytosine was the sole methylated base detected. 3. Chromatographic separations of 3-methylcytosine from 5-methylcytosine and purines are discussed.

MeSH Terms
Carbon Isotopes Chromatography, Paper Cytosine/analysis DNA, Neoplasm/metabolism HeLa Cells/metabolism Humans Methionine/metabolism Methylation Purines/analysis
Chemicals
Carbon Isotopes DNA, Neoplasm Purines Cytosine Methionine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lawley P D
Crathorn A R
Shah S A
Smith B A
References (12)
12 references, click to expand
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  10. Methylation of ribonucleic acid by the carcinogens dimethyl sulphate, N-methyl-N-nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine. Comparisons of chemical analyses at the nucleoside and base levels.
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1280133
Published
1972-06-00
Pages
133-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1173577
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1280133 PMC Full Text View journal details
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