Home LiteratureArticle Details
PMID: 1069283 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Colicin E2 is DNA endonuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 3989-93

Schaller K, Nomura M

Abstract

Colicin E2 purified by conventional methods contains a tightly bound low-molecular-weight protein, as has been found with purified colicin E3 [Jakes,N.&Zinder,N.D.(1974) Proc. Natl. Acad. Sci. USA 71, 3380-3384]. Such E2 preparations do not cause DNA cleavage in vitro. After separation from the low-molecular-weight protein, colicin E2 retained the original in vivo killing activity, and in addition showed a high activity in vitro in cleaving various DNA molecules, such as a ColE1 hybrid plasmid and DNAs from Escherichia coli, lambda phage, chiX174 phage, and simian virus 40. The low-molecular-weight protein ("E2-immunity protein") specifically prevented this in vitro DNA cleavage reaction, i.e., had an "immunity function." The results demonstrate that colicin E2 itself is a DNA endonuclease and explain the in vivo effects caused by E2 in sensitive cells as well as the mechanism of immunity in E2-colicinogenic cells.

MeSH Terms
Colicins/antagonists & inhibitors,isolation & purification,metabolism DNA/metabolism DNA, Bacterial/metabolism DNA, Circular/metabolism Endonucleases/metabolism Guanidines Molecular Weight
Chemicals
Colicins DNA, Bacterial DNA, Circular Guanidines DNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schaller K
Nomura M
References (29)
29 references, click to expand
  1. Degradation of ribosomal RNA from Escherichia coli induced by colicine E2.
    Biochim Biophys Acta. 1966 Jun 22;119(3):636-8 PMID: 5336314
  2. Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli.
    J Mol Biol. 1975 Nov 5;98(3):551-64 PMID: 1104875
  3. Biochemical construction and selection of hybrid plasmids containing specific segments of the Escherichia coli genome.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4361-5 PMID: 1105581
  4. Sedimentation analysis of DNA degradation products resulting from the action of colicin E2 on Escherichia coli.
    Biochim Biophys Acta. 1970 Aug 8;213(2):320-34 PMID: 4927488
  5. The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease.
    J Biol Chem. 1962 Mar;237:819-28 PMID: 14463751
  6. Inactivation of ribosomes by colicin E3 in vitro: Requirement for 50 S ribosomal subunits.
    FEBS Lett. 1972 Apr 15;22(1):73-75 PMID: 11946564
  7. Inactivation of infectious deoxyribonucleic acid of bacteriophage phi chi X 174 by colicin E2.
    Biochim Biophys Acta. 1973 Oct 26;324(3):305-8 PMID: 4202716
  8. Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells.
    Nat New Biol. 1971 Dec 1;234(48):133-7 PMID: 4332178
  9. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo.
    Proc Natl Acad Sci U S A. 1971 May;68(5):964-8 PMID: 4930244
  10. Colicins and colicinogenic factors.
    Symp Soc Exp Biol. 1958;12:104-22 PMID: 13580865
  11. Hybridization of labeled RNA to DNA in agarose gels.
    Nucleic Acids Res. 1975 Oct;2(10):1911-29 PMID: 1103090
  12. The action of colicin E2 on supercoiled lambda DNA.II. Experiments in vitro.
    Biochemistry. 1975 May 20;14(10):2058-63 PMID: 167801
  13. Specific inhibition of cell division by colicin E 2 without degradation of deoxyribonucleic acid in a new colicin sensitivity mutant of Escherichia coli.
    J Bacteriol. 1972 May;110(2):485-93 PMID: 4553833
  14. Purification and properties of colicin E3 immunity protein.
    J Biol Chem. 1974 Jan 25;249(2):438-44 PMID: 4588566
  15. Highly purified colicin E3 contains immunity protein.
    Proc Natl Acad Sci U S A. 1974 Sep;71(9):3380-4 PMID: 4215078
  16. Mechanism of colicin E2-induced DNA degradation in Escherichia coli.
    Biochim Biophys Acta. 1970 Feb 18;199(2):330-9 PMID: 4907332
  17. Role for endonuclease I in the transmission process of colicin E 2 .
    Nat New Biol. 1972 Feb 16;235(59):199-203 PMID: 4551899
  18. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  19. Purification and characterization of colicin E2 and colicin E3.
    J Biol Chem. 1967 Nov 25;242(22):5360-8 PMID: 4965137
  20. The action of colicin E2 on supercoiled lambda DNA. I. Experiments in vivo.
    Biochemistry. 1975 May 20;14(10):2051-7 PMID: 1148158
  21. Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 May;68(5):959-63 PMID: 4930243
  22. Inactivation of ribosomes in vitro by colicin E 3 and its mechanism of action.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):549-52 PMID: 4551976
  23. Interaction between colicin E2 and DNA in vitro.
    FEBS Lett. 1972 Jun 15;23(2):241-3 PMID: 4564150
  24. Existence of some substances which react to colicin E2.
    Biochim Biophys Acta. 1974 Dec 20;374(3):426-30 PMID: 4611501
  25. Interaction of colicins with bacterial cells. II. Specific alteration of Escherichia coli ribosomes induced by colicin E3 in vivo.
    J Mol Biol. 1967 Jun 14;26(2):181-95 PMID: 5340611
  26. Inactivation of ribosomes in vitro by colicin E 3 .
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421-5 PMID: 4944624
  27. In vitro synthesis of the E3 immunity protein directed by Col E3 plasmid deoxyribonucleic acid.
    J Biol Chem. 1975 Feb 10;250(3):1123-31 PMID: 803499
  28. SOME PHYSICAL PROPERTIES OF BACTERIOPHAGE R17 AND ITS RIBONUCLEIC ACID.
    J Mol Biol. 1964 Apr;8:496-507 PMID: 14153522
  29. Colicins and related bacteriocins.
    Annu Rev Microbiol. 1967;21:257-84 PMID: 4860260
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.11.3989
Published
1976-11-00
Pages
3989-93
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431295
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.11.3989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2