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PMID: 10698933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Interaction between the tobacco mosaic virus movement protein and host cell pectin methylesterases is required for viral cell-to-cell movement.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 913-20

Chen MH, Sheng J, Hind G, Handa AK, Citovsky V

Abstract

Virus-encoded movement protein (MP) mediates cell-to-cell spread of tobacco mosaic virus (TMV) through plant intercellular connections, the plasmodesmata. The molecular pathway by which TMV MP interacts with the host cell is largely unknown. To understand this process better, a cell wall-associated protein that specifically binds the viral MP was purified from tobacco leaf cell walls and identified as pectin methylesterase (PME). In addition to TMV MP, PME is recognized by MPs of turnip vein clearing virus (TVCV) and cauliflower mosaic virus (CaMV). The use of amino acid deletion mutants of TMV MP showed that its domain was necessary and sufficient for association with PME. Deletion of the PME-binding region resulted in inactivation of TMV cell-to-cell movement.

MeSH Terms
Amino Acid Sequence Binding Sites Carboxylic Ester Hydrolases/metabolism Molecular Sequence Data Plant Viral Movement Proteins Plants, Toxic Protein Binding Sequence Alignment Tobacco/metabolism,virology Tobacco Mosaic Virus/physiology Viral Proteins/metabolism Virus Replication
Chemicals
Plant Viral Movement Proteins Viral Proteins Carboxylic Ester Hydrolases pectinesterase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chen M H
Department of Biochemistry, State University of New York, Stony Brook, NY 11794-5215, USA.
Sheng J
Hind G
Handa A K
Citovsky V
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.913
Published
2000-03-01
Pages
913-20
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305631
Subset
IM
Grants
NIGMS NIH HHS · R01 GM050224 · United States
NIGMS NIH HHS · GM50224 · United States
External Links
PubMed source DOI: 10.1093/emboj/19.5.913 PMC Full Text View journal details
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