Nucleotide sequence of tobacco mosaic virus RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 19 ·1982-10-00 ·Pages 5818-22

Goelet P, Lomonossoff GP, Butler PJ, Akam ME, Gait MJ, Karn J

Abstract

Oligonucleotide primers have been used to generate a cDNA library covering the entire tobacco mosaic virus (TMV) RNA sequence. Analysis of these clones has enabled us to complete the viral RNA sequence and to study its variability within a viral population. The positive strand coding sequence starts 69 nucleotides from the 5' end with a reading frame for a protein of Mr 125,941 and terminates with UAG. Readthrough of this terminator would give rise to a protein of Mr 183,253. Overlapping the terminal five codons of this readthrough reading frame is a second reading frame coding for a protein of Mr 29,987. This gene terminates two nucleotides before the initiator codon of the coat protein gene. Potential signal sequences responsible for the capping and synthesis of the coat protein and Mr 29,987 protein mRNAs have been identified. Similar sequences within these reading frames may be used in the expression of sets of proteins that share COOH-terminal sequences.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA/analysis RNA, Messenger/genetics RNA, Viral/genetics Tobacco Mosaic Virus/genetics Viral Proteins/genetics

Chemicals

RNA, Messenger RNA, Viral Viral Proteins DNA

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Goelet P

Lomonossoff G P

Butler P J

Akam M E

Gait M J

Karn J

References (25)

25 references, click to expand

Mammalian peptide chain termination. II. Codon specificity and GTPase activity of release factor.
Proc Natl Acad Sci U S A. 1971 Mar;68(3):619-24 PMID: 5276771
Nucleotide sequence of Moloney murine leukaemia virus.
Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.
Nucleic Acids Res. 1977 Apr;4(4):1135-58 PMID: 866189
The nucleotide sequence at the origin for assembly on tobacco mosaic virus RNA.
Cell. 1977 Jul;11(3):463-82 PMID: 884732
Characterization and in vitro translation of the RNAs from less-than-full-length, virus-related, nucleoprotein rods present in tobacco mosaic virus preparations.
Virology. 1977 Aug;81(1):160-9 PMID: 888358
Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.
J Biol Chem. 1978 Apr 10;253(7):2483-95 PMID: 75887
Leaky UAG termination codon in tobacco mosaic virus RNA.
Nature. 1978 Mar 30;272(5652):469-71 PMID: 634374
Nucleotide sequence at the 5' extremity of tobacco-mosaic-virus RNA. 2. The coding region (nucleotides 69-236).
Eur J Biochem. 1978 Mar 15;84(2):521-31 PMID: 639801
Nucleotide sequence heterogeneity of an RNA phage population.
Cell. 1978 Apr;13(4):735-44 PMID: 657273
Quantized incorporation of RNA during assembly of tobacco mosaic virus from protein disks.
J Mol Biol. 1978 Dec 25;126(4):877-82 PMID: 745247
Translation of fragmented viral RNA in vitro: initiation at multiple sites.
FEBS Lett. 1979 Apr 1;100(1):195-9 PMID: 437104
Sequence of 1000 nucleotides at the 3' end of tobacco mosaic virus RNA.
Nucleic Acids Res. 1979 Apr;6(4):1287-308 PMID: 109810
Assembly of tobacco mosaic virus: elongation towards the 3'-hydroxyl terminus of the RNA.
FEBS Lett. 1980 May 5;113(2):271-4 PMID: 7389898
Usage of the three termination codons: compilation and analysis of the known eukaryotic and prokaryotic translation termination sequences.
Mol Gen Genet. 1981;182(3):430-9 PMID: 6946272
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes.
Nucleic Acids Res. 1981 Oct 24;9(20):5233-52 PMID: 7301588
Complete nucleotide sequence of brome mosaic virus RNA3.
J Mol Biol. 1981 Nov 25;153(1):23-38 PMID: 7338913
Tobacco mosaic virus induces the synthesis of a family of 3' coterminal messenger RNAs and their complements.
J Mol Biol. 1982 Jan 25;154(3):541-50 PMID: 7077671
Two leaky termination codons in AMV RNA 1.
FEBS Lett. 1980 Aug 25;118(1):67-71 PMID: 7409194
A new computer method for the storage and manipulation of DNA gel reading data.
Nucleic Acids Res. 1980 Aug 25;8(16):3673-94 PMID: 7433103
Nucleotide sequences at the 5'-termini of the alfalfa mosaic virus RNAs and the intercistronic junction in RNA 3.
Nucleic Acids Res. 1980 Dec 11;8(23):5635-47 PMID: 6927843
Messenger RNA for the coat protein of tobacco mosaic virus.
Nature. 1976 Apr 29;260(5554):759-64 PMID: 1264250
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
UGA suppression by normal tRNA Trp in Escherichia coli: codon context effects.
Nucleic Acids Res. 1981 Feb 25;9(4):983-91 PMID: 7015288
Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
A system for shotgun DNA sequencing.
Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.19.5818

Published

1982-10-00

Pages

5818-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC347001

Subset

Databases

GENBANK

J02415

External Links

PubMed source DOI: 10.1073/pnas.79.19.5818 PMC Full Text View journal details

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