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PMID: 10698948 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cap-dependent deadenylation of mRNA.

The EMBO journal ·Vol. 19 ·No. 5 ·2000-03-01 ·Pages 1079-86

Dehlin E, Wormington M, Körner CG, Wahle E

Abstract

Poly(A) tail removal is often the initial and rate-limiting step in mRNA decay and is also responsible for translational silencing of maternal mRNAs during oocyte maturation and early development. Here we report that deadenylation in HeLa cell extracts and by a purified mammalian poly(A)-specific exoribonuclease, PARN (previously designated deadenylating nuclease, DAN), is stimulated by the presence of an m(7)-guanosine cap on substrate RNAs. Known cap-binding proteins, such as eIF4E and the nuclear cap-binding complex, are not detectable in the enzyme preparation, and PARN itself binds to m(7)GTP-Sepharose and is eluted specifically with the cap analog m(7)GTP. Xenopus PARN is known to catalyze mRNA deadenylation during oocyte maturation. The enzyme is depleted from oocyte extract with m(7)GTP-Sepharose, can be photocross-linked to the m(7)GpppG cap and deadenylates m(7)GpppG-capped RNAs more efficiently than ApppG-capped RNAs both in vitro and in vivo. These data provide additional evidence that PARN is responsible for deadenylation during oocyte maturation and suggest that interactions between 5' cap and 3' poly(A) tail may integrate translational efficiency with mRNA stability.

MeSH Terms
Adenosine Monophosphate Exoribonucleases/chemistry,isolation & purification,metabolism HeLa Cells Humans Protein Biosynthesis RNA, Messenger/chemistry,metabolism
Chemicals
RNA, Messenger Adenosine Monophosphate Exoribonucleases poly A specific exoribonuclease
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dehlin E
Institut für Biochemie, Universität Halle-Wittenberg, D-06099 Halle.
Wormington M
Körner C G
Wahle E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.5.1079
Published
2000-03-01
Pages
1079-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305646
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.5.1079 PMC Full Text View journal details
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