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PMID: 10716708 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

SAC3 may link nuclear protein export to cell cycle progression.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 7 ·2000-03-28 ·Pages 3224-9

Jones AL, Quimby BB, Hood JK, Ferrigno P, Keshava PH, Silver PA, Corbett AH

Abstract

Selective movement of proteins between the nucleus and the cytoplasm is a regulatory mechanism exploited extensively by the eukaryotic cell. We have identified the evolutionarily conserved Sac3 protein, which was implicated previously in the regulation of mitosis [Bauer, A. & Kölling, R. (1996) J. Cell Sci. 109, 1575-1583] as a novel mediator of nuclear protein export. We show that Sac3p is localized to the nuclear pore, where it interacts with nucleoporins. Loss of SAC3 function results in a block in nuclear export of a nuclear export signal-containing reporter protein. Our results also demonstrate that SAC3 interacts genetically with the nuclear protein export factors Crm1p/Xpo1p and Yrb2p. Taken together, these data indicate a link between nuclear protein export and transition through the cell cycle.

MeSH Terms
Amino Acid Sequence Biological Transport Calcium-Binding Proteins Cell Cycle Fluorescent Antibody Technique, Indirect Fungal Proteins/metabolism Gene Deletion Green Fluorescent Proteins Luminescent Proteins/genetics Molecular Sequence Data Nuclear Pore Complex Proteins Nuclear Proteins/genetics,metabolism Nucleocytoplasmic Transport Proteins Porins Protein Binding Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid
Chemicals
Calcium-Binding Proteins Fungal Proteins Luminescent Proteins NSP1 protein, S cerevisiae Nuclear Pore Complex Proteins Nuclear Proteins Nucleocytoplasmic Transport Proteins Porins Recombinant Fusion Proteins SAC3 protein, S cerevisiae Saccharomyces cerevisiae Proteins Green Fluorescent Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Jones A L
Department of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
Quimby B B
Hood J K
Ferrigno P
Keshava P H
Silver P A
Corbett A H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.050432997
Published
2000-03-28
Pages
3224-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC16220
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.050432997 PMC Full Text View journal details
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