Home LiteratureArticle Details
PMID: 10716938 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Fission yeast switches mating type by a replication-recombination coupled process.

The EMBO journal ·Vol. 19 ·No. 6 ·2000-03-15 ·Pages 1389-96

Arcangioli B, de Lahondès R

Abstract

Fission yeast exhibits a homothallic life cycle, in which the mating type of the cell mitotically alternates in a highly regulated fashion. Pedigree analysis of dividing cells has shown that only one of the two sister cells switches mating type. It was shown recently that a site- and strand-specific DNA modification at the mat1 locus precedes mating-type switching. By tracking the fate of mat1 DNA throughout the cell cycle with a PCR assay, we identified a novel DNA intermediate of mating-type switching in S-phase. The time and rate of appearance and disappearance of this DNA intermediate are consistent with a model in which mating-type switching occurs through a replication-recombination coupled pathway. Such a process provides experimental evidence in support of a copy choice recombination model in Schizosaccharomyces pombe mating-type switching and is reminiscent of the sister chromatid recombination used to complete replication in the presence of certain types of DNA damage.

MeSH Terms
DNA Replication/genetics DNA, Fungal/genetics G2 Phase Gene Conversion/genetics Genes, Fungal/genetics Genes, Mating Type, Fungal Genes, Switch/genetics Genomic Imprinting/genetics Kinetics Models, Genetic Mutation/genetics Polymerase Chain Reaction S Phase Schizosaccharomyces/cytology,genetics,growth & development Sister Chromatid Exchange/genetics
Chemicals
DNA, Fungal
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Arcangioli B
Unite des Virus Oncogenes, URA 1644 du CNRS, Departement des Biotechnologies, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France.
de Lahondès R
References (50)
50 references, click to expand
  1. Analysis of DNA repair pathways of Schizosaccharomyces pombe by means of swi-rad double mutants.
    Mutat Res. 1993 Jun;294(1):59-67 PMID: 7683759
  2. The shuffling of a mortal coil.
    Nat Genet. 1999 May;22(1):4-6 PMID: 10319847
  3. DNA synthesis dependent on genetic recombination: characterization of a reaction catalyzed by purified bacteriophage T4 proteins.
    Cell. 1986 Dec 5;47(5):793-806 PMID: 3022939
  4. The mechanism of fission yeast mating type interconversion: seal/replicate/cleave model of replication across the double-stranded break site at mat1.
    Genetics. 1991 Mar;127(3):489-96 PMID: 2016051
  5. DNA polymerase-alpha is essential for mating-type switching in fission yeast.
    Nature. 1993 Jan 21;361(6409):271-3 PMID: 8423854
  6. Crossing over and Gene Rearrangement in Flowering Plants.
    Genetics. 1933 Jul;18(4):388-413 PMID: 17246698
  7. Double-strand break repair in yeast requires both leading and lagging strand DNA polymerases.
    Cell. 1999 Feb 5;96(3):415-24 PMID: 10025407
  8. The msh2 gene of Schizosaccharomyces pombe is involved in mismatch repair, mating-type switching, and meiotic chromosome organization.
    Mol Cell Biol. 1999 Jan;19(1):241-50 PMID: 9858548
  9. The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion.
    Curr Genet. 1993 Feb;23(2):184-6 PMID: 8431959
  10. Sister chromatid exchanges induced in Chinese hamster cells by UV irradiation of different stages of the cell cycle: the necessity for cells to pass through S.
    Mutat Res. 1974 Oct;25(1):73-81 PMID: 4473707
  11. Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease.
    Nature. 1993 Apr 29;362(6423):860-2 PMID: 8479526
  12. Switching genes in Schizosaccharomyces pombe.
    Curr Genet. 1985;9(5):325-31 PMID: 24719968
  13. DNA recombination: the replication connection.
    Trends Biochem Sci. 1999 Jul;24(7):271-5 PMID: 10390616
  14. The pedigree pattern of mating-type switching in Schizosaccharomyces pombe.
    Curr Genet. 1984 Apr;8(3):205-10 PMID: 24177740
  15. The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes.
    Nucleic Acids Res. 1992 May 11;20(9):2271-8 PMID: 1317550
  16. Initiation of meiotic recombination by double-strand DNA breaks in S. pombe.
    Cell. 1986 Aug 29;46(5):725-31 PMID: 3742597
  17. Some of the swi genes of Schizosaccharomyces pombe also have a function in the repair of radiation damage.
    Curr Genet. 1989 Aug;16(2):89-94 PMID: 2598273
  18. Frequency of mating-type switching in homothallic fission yeast.
    Nature. 1977 Mar 10;266(5598):172-4 PMID: 859590
  19. Specific cleavage of model recombination and repair intermediates by the yeast Rad1-Rad10 DNA endonuclease.
    Science. 1994 Sep 30;265(5181):2082-5 PMID: 8091230
  20. A novel switch-activating site (SAS1) and its cognate binding factor (SAP1) required for efficient mat1 switching in Schizosaccharomyces pombe.
    EMBO J. 1991 Oct;10(10):3025-32 PMID: 1915277
  21. The developmental fate of fission yeast cells is determined by the pattern of inheritance of parental and grandparental DNA strands.
    EMBO J. 1990 May;9(5):1407-15 PMID: 2328720
  22. Rearrangements of the transposable mating-type cassettes of fission yeast.
    EMBO J. 1984 Mar;3(3):603-10 PMID: 6325178
  23. Gene activation by copy transposition in mating-type switching of a homothallic fission yeast.
    Curr Genet. 1981 Apr;3(1):5-12 PMID: 24189946
  24. Holliday junctions accumulate in replication mutants via a RecA homolog-independent mechanism.
    Cell. 1997 Jul 11;90(1):87-96 PMID: 9230305
  25. Elimination of replication block protein Fob1 extends the life span of yeast mother cells.
    Mol Cell. 1999 Apr;3(4):447-55 PMID: 10230397
  26. Four mating-type genes control sexual differentiation in the fission yeast.
    EMBO J. 1988 May;7(5):1537-47 PMID: 2900761
  27. Directionality of fission yeast mating-type interconversion is controlled by the location of the donor loci.
    Genetics. 1993 Aug;134(4):1045-54 PMID: 8375648
  28. The fission yeast rad22 gene, having a function in mating-type switching and repair of DNA damages, encodes a protein homolog to Rad52 of Saccharomyces cerevisiae.
    Nucleic Acids Res. 1993 Dec 25;21(25):5940-4 PMID: 8290356
  29. Recombinational repair of gaps in DNA is asymmetric in Ustilago maydis and can be explained by a migrating D-loop model.
    Proc Natl Acad Sci U S A. 1996 May 28;93(11):5419-24 PMID: 8643590
  30. The essential role of recombination in phage T4 growth.
    Annu Rev Genet. 1987;21:347-71 PMID: 3327469
  31. The rad16 gene of Schizosaccharomyces pombe: a homolog of the RAD1 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1994 Mar;14(3):2029-40 PMID: 8114734
  32. Stable DNA replication: interplay between DNA replication, homologous recombination, and transcription.
    Microbiol Mol Biol Rev. 1997 Jun;61(2):212-38 PMID: 9184011
  33. Requirement of mismatch repair genes MSH2 and MSH3 in the RAD1-RAD10 pathway of mitotic recombination in Saccharomyces cerevisiae.
    Genetics. 1996 Mar;142(3):727-36 PMID: 8849883
  34. swi6, a gene required for mating-type switching, prohibits meiotic recombination in the mat2-mat3 "cold spot" of fission yeast.
    Genetics. 1991 Dec;129(4):1033-42 PMID: 1783290
  35. Genes required for initiation and resolution steps of mating-type switching in fission yeast.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3481-5 PMID: 6587363
  36. Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing.
    EMBO J. 1989 Jan;8(1):269-76 PMID: 2714252
  37. The switching gene swi6 affects recombination and gene expression in the mating-type region of Schizosaccharomyces pombe.
    Mol Gen Genet. 1992 Jun;233(3):436-42 PMID: 1620099
  38. Replication-dependent sister chromatid recombination in rad1 mutants of Saccharomyces cerevisiae.
    Genetics. 1993 Mar;133(3):469-87 PMID: 8454200
  39. Differentiated parental DNA strands confer developmental asymmetry on daughter cells in fission yeast.
    Nature. 1987 Apr 2-8;326(6112):466-70 PMID: 3561486
  40. Intermediates of recombination during mating type switching in Saccharomyces cerevisiae.
    EMBO J. 1990 Mar;9(3):663-73 PMID: 2178924
  41. A site- and strand-specific DNA break confers asymmetric switching potential in fission yeast.
    EMBO J. 1998 Aug 3;17(15):4503-10 PMID: 9687516
  42. Complementation of the DNA repair-deficient swi10 mutant of fission yeast by the human ERCC1 gene.
    Nucleic Acids Res. 1997 Jul 15;25(14):2823-7 PMID: 9207030
  43. Two pathways for removal of nonhomologous DNA ends during double-strand break repair in Saccharomyces cerevisiae.
    Mol Cell Biol. 1997 Nov;17(11):6765-71 PMID: 9343441
  44. Extrachromosomal rDNA circles--a cause of aging in yeast.
    Cell. 1997 Dec 26;91(7):1033-42 PMID: 9428525
  45. Role of Saccharomyces cerevisiae Msh2 and Msh3 repair proteins in double-strand break-induced recombination.
    Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9214-9 PMID: 9256462
  46. A subthreshold level of DNA topoisomerases leads to the excision of yeast rDNA as extrachromosomal rings.
    Cell. 1989 Jun 16;57(6):975-85 PMID: 2544296
  47. DNA double-strand breaks caused by replication arrest.
    EMBO J. 1997 Jan 15;16(2):430-8 PMID: 9029161
  48. A mutated swi4 gene causes duplications in the mating-type region of Schizosaccharomyces pombe.
    Curr Genet. 1990 Dec;18(6):501-9 PMID: 2076550
  49. cdc25+ functions as an inducer in the mitotic control of fission yeast.
    Cell. 1986 Apr 11;45(1):145-53 PMID: 3955656
  50. Orientation of DNA replication establishes mating-type switching pattern in S. pombe.
    Nature. 1999 Jul 8;400(6740):181-4 PMID: 10408447
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/19.6.1389
Published
2000-03-15
Pages
1389-96
Language
English
Region
England
NLM ID
8208664
PMCID
PMC305679
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/19.6.1389 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2