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PMID: 10725385 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Arabidopsis RelA/SpoT homologs implicate (p)ppGpp in plant signaling.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 97 ·No. 7 ·2000-03-28 ·Pages 3747-52

van der Biezen EA, Sun J, Coleman MJ, Bibb MJ, Jones JD

Abstract

Arabidopsis RPP5 is a member of a large class of pathogen resistance genes encoding nucleotide-binding sites and leucine-rich repeat domains. Yeast two-hybrid analysis showed that RPP5 specifically interacts with At-RSH1, an Arabidopsis RelA/SpoT homolog. In Escherichia coli, RelA and SpoT determine the level of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp), which are the effector nucleotides of the bacterial stringent response. Functional analysis in E. coli and in Streptomyces coelicolor A3 (2) showed that At-RSH1 confers phenotypes associated with (p)ppGpp synthesis. We characterized two additional Arabidopsis RelA/SpoT homologs, At-RSH2 and At-RSH3. At-RSH genes may regulate a rapid plant (p)ppGpp-mediated response to pathogens and other stresses.

MeSH Terms
Arabidopsis/enzymology,genetics,metabolism Arabidopsis Proteins Escherichia coli/metabolism Guanosine Pentaphosphate/genetics,metabolism Ligases/genetics,metabolism Molecular Sequence Data Mutation Phenotype Plant Proteins/chemistry,genetics,metabolism Signal Transduction Streptomyces/metabolism Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins Plant Proteins RSH1 protein, Arabidopsis RSH2 protein, Arabidopsis Guanosine Pentaphosphate Ligases guanosine 3',5'-polyphosphate synthetases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van der Biezen E A
The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom.
Sun J
Coleman M J
Bibb M J
Jones J D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.060392397
Published
2000-03-28
Pages
3747-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC16311
Subset
IM
Databases
GENBANK
AF225702, AF225703, AF225704
External Links
PubMed source DOI: 10.1073/pnas.060392397 PMC Full Text View journal details
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