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PMID: 9294445 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) plays a conditional role in antibiotic production and morphological differentiation.

Journal of bacteriology ·Vol. 179 ·No. 18 ·1997-09-00 ·Pages 5854-61

Chakraburtty R, Bibb M

Abstract

Deletion of most of the coding region of the ppGpp synthetase gene (relA) of Streptomyces coelicolor A3(2) resulted in loss of ppGpp synthesis, both upon entry into stationary phase under conditions of nitrogen limitation and following amino acid starvation during exponential growth, but had no effect on growth rate. The relA mutant, which showed continued rRNA synthesis upon amino acid depletion (the relaxed response), failed to produce the antibiotics undecylprodigiosin (Red) and actinorhodin (Act) under conditions of nitrogen limitation. The latter appears to reflect diminished transcription of pathway-specific regulatory genes for Red and Act production, redD and actII-ORF4, respectively. In addition to the changes in secondary metabolism, the relA mutant showed a marked delay in the onset and extent of morphological differentiation, resulting in a conspicuously altered colony morphology.

MeSH Terms
Anthraquinones/metabolism Bacterial Proteins Gene Expression Regulation, Bacterial Genetic Complementation Test Guanosine Tetraphosphate/biosynthesis Ligases/physiology Nitrogen/metabolism Prodigiosin/analogs & derivatives,biosynthesis RNA, Bacterial/biosynthesis Streptomyces/cytology,enzymology,genetics Trans-Activators/genetics Transcription, Genetic
Chemicals
Anthraquinones Bacterial Proteins RNA, Bacterial Trans-Activators redD protein, Streptomyces coelicolor Guanosine Tetraphosphate undecylprodigiosin Ligases guanosine 3',5'-polyphosphate synthetases actinorhodin Nitrogen Prodigiosin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chakraburtty R
Department of Genetics, John Innes Centre, Norwich Research Park, Colney, United Kingdom.
Bibb M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.18.5854-5861.1997
Published
1997-09-00
Pages
5854-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179477
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.179.18.5854-5861.1997 PMC Full Text View journal details
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