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PMID: 7642495 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two genes involved in the phase-variable phi C31 resistance mechanism of Streptomyces coelicolor A3(2).

Journal of bacteriology ·Vol. 177 ·No. 16 ·1995-08-00 ·Pages 4681-9

Bedford DJ, Laity C, Buttner MJ

Abstract

The phage growth limitation (Pgl) system of Streptomyces coelicolor confers resistance to phi C31 and its homoimmune phages. The positions of the pgl genes within a 16-kb clone of S. coelicolor DNA were defined by subcloning, insertional inactivation, and deletion mapping. Nucleotide sequencing and functional analysis identified two genes, pglY and pglZ, required for the Pgl+ (phage-resistant) phenotype. pglY and pglZ, which may be translationally coupled, are predicted to encode proteins with M(r)S of 141,000 and 104,000, respectively. Neither protein shows significant similarity to other known proteins, but PglY has a putative ATP/GTP binding motif. The pglY and pglZ genes are cotranscribed from a single promoter which appears to be constitutive and is not induced by phage infection.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Bacteriophages/pathogenicity Base Sequence Carrier Proteins Cloning, Molecular Genes, Bacterial/genetics Intracellular Signaling Peptides and Proteins Molecular Sequence Data Mutagenesis Open Reading Frames/genetics Operon/genetics Phenotype Plasmids/genetics Protein Biosynthesis Recombination, Genetic Restriction Mapping Sequence Deletion Streptomyces/genetics,virology Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins Intracellular Signaling Peptides and Proteins PglY protein, Streptomyces coelicolor PglZ protein, Streptomyces coelicolor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bedford D J
Department of Genetics, John Innes Centre, Colney, Norwich, United Kingdom.
Laity C
Buttner M J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.16.4681-4689.1995
Published
1995-08-00
Pages
4681-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177233
Subset
IM
Databases
GENBANK
L37531
External Links
PubMed source DOI: 10.1128/jb.177.16.4681-4689.1995 PMC Full Text View journal details
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