Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis.
Streptomyces avermitilis contains a unique restriction system that restricts plasmid DNA containing N6-methyladenine or 5-methylcytosine. Shuttle vectors isolated from Escherichia coli RR1 or plasmids isolated from modification-proficient Streptomyces spp. cannot be directly introduced into S. avermitilis. This restriction barrier can be overcome by first transferring plasmids into Streptomyces lividans or a modification-deficient E. coli strain and then into S. avermitilis. The transformation frequency was reduced greater than 1,000-fold when plasmid DNA was modified by dam or TaqI methylases to contain N6-methyladenine or by AluI, HhaI, HphI methylases to contain 5-methylcytosine. Methyl-specific restriction appears to be common in Streptomyces spp., since either N6-methyladenine-specific or 5-methylcytosine-specific restriction was observed in seven of nine strains tested.
30 references, click to expand
-
Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli.J Bacteriol. 1972 Jun;110(3):1135-46 PMID: 4555406
-
Inhibition of restriction in Streptomyces clavuligerus by heat treatment.J Gen Microbiol. 1986 Oct;132(10):2945-7 PMID: 3040892
-
Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation.J Mol Biol. 1977 Jul;114(1):153-68 PMID: 20509
-
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene. 1977;2(2):95-113 PMID: 344137
-
Avermectins, new family of potent anthelmintic agents: producing organism and fermentation.Antimicrob Agents Chemother. 1979 Mar;15(3):361-7 PMID: 464561
-
A rapid boiling method for the preparation of bacterial plasmids.Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
-
pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.Mol Gen Genet. 1982;185(2):223-8 PMID: 6283316
-
Cloning of antibiotic resistance and nutritional genes in streptomycetes.J Bacteriol. 1982 Aug;151(2):668-77 PMID: 6284706
-
Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction.Gene. 1982 Nov;20(1):51-62 PMID: 6298066
-
The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene.Nucleic Acids Res. 1983 Feb 11;11(3):837-51 PMID: 6300769
-
Plasmids, recombination and chromosome mapping in Streptomyces lividans 66.J Gen Microbiol. 1983 Jul;129(7):2257-69 PMID: 6631413
-
Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581.J Bacteriol. 1984 Jan;157(1):314-7 PMID: 6317655
-
pFJ265, a new cloning vehicle for Streptomyces.Plasmid. 1984 Jan;11(1):92-5 PMID: 6324262
-
Restriction of bacteriophage plaque formation in Streptomyces spp.J Bacteriol. 1984 Aug;159(2):499-504 PMID: 6086574
-
Production of 'hybrid' antibiotics by genetic engineering.Nature. 1985 Apr 18-24;314(6012):642-4 PMID: 3857464
-
Restriction and modification enzymes and their recognition sequences.Nucleic Acids Res. 1985;13 Suppl:r165-200 PMID: 2987885
-
New derivatives of the Streptomyces temperate phage phi C31 useful for the cloning and functional analysis of Streptomyces DNA.Gene. 1985;34(2-3):283-92 PMID: 2989111
-
Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate.Nucleic Acids Res. 1985 Aug 26;13(16):5727-46 PMID: 2994011
-
Restriction-modification systems in Streptomyces antibioticus.Can J Microbiol. 1985 Oct;31(10):942-6 PMID: 2998580
-
Isolation and characterization of the Streptomyces cattleya temperate phage TG1.Gene. 1985;39(1):11-6 PMID: 3000891
-
Mycoplasma restriction: identification of a new type of restriction specificity for DNA containing 5-methylcytosine.J Bacteriol. 1986 Jan;165(1):219-25 PMID: 3001023
-
The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA.Plasmid. 1986 May;15(3):199-209 PMID: 3012613
-
Escherichia coli K-12 restricts DNA containing 5-methylcytosine.Proc Natl Acad Sci U S A. 1986 Dec;83(23):9070-4 PMID: 3024165
-
Characterization of the Streptomyces plasmid pVE1.Plasmid. 1986 Nov;16(3):182-94 PMID: 3027726
-
Characterization of suppressible mutations in the viomycin phosphotransferase gene of the Streptomyces enteric plasmid pVE138.J Bacteriol. 1987 Mar;169(3):1325-7 PMID: 3029035
-
N4-methylcytosine as a minor base in bacterial DNA.J Bacteriol. 1987 Mar;169(3):939-43 PMID: 3029036
-
Specificity of restriction endonucleases and methylases--a review.Gene. 1986;47(1):1-153 PMID: 3030890
-
Highly transformable mutants of Streptomyces fradiae defective in several restriction systems.Mol Gen Genet. 1987 Mar;206(3):393-400 PMID: 3473276
-
Hemimethylation prevents DNA replication in E. coli.Cell. 1987 Sep 25;50(7):1071-9 PMID: 3304662
-
Formation and reversion of Streptomycete protoplasts: cultural condition and morphological study.J Gen Microbiol. 1974 Feb;80(2):389-400 PMID: 4207870
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
