Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581.

Journal of bacteriology ·Vol. 157 ·No. 1 ·1984-01-00 ·Pages 314-7

Larson JL, Hershberger CL

Abstract

The replicon of the Streptomyces plasmid SCP2 was located on a 5.9-kilobase EcoRI-SalI restriction fragment. The SCP2 replicon was combined with Escherichia coli plasmid pBR322 and genes specifying neomycin resistance and thiostrepton resistance in streptomycetes to construct shuttle vectors that are useful for cloning in E. coli and streptomycetes.

MeSH Terms

Chromosome Mapping Cloning, Molecular/methods DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics Genetic Vectors Plasmids Replicon Streptomyces/genetics

Chemicals

DNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Larson J L

Hershberger C L

References (13)

13 references, click to expand

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Restriction and modification enzymes and their recognition sequences.
Nucleic Acids Res. 1982 Mar 11;10(5):r117-44 PMID: 6280143
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Physical analysis of antibiotic-resistance genes from Streptomyces and their use in vector construction.
Gene. 1982 Nov;20(1):51-62 PMID: 6298066
Development of cloning vehicles from the Streptomyces plasmid pFJ103.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.157.1.314-317.1984

Published

1984-01-00

Pages

314-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215173

Subset

External Links

PubMed source DOI: 10.1128/jb.157.1.314-317.1984 PMC Full Text View journal details

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Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581.

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