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PMID: 3025560 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2).

Molecular & general genetics : MGG ·Vol. 205 ·No. 1 ·1986-10-00 ·Pages 66-73

Malpartida F, Hopwood DA

Abstract

We determined the physical and transcriptional organisation of the set of previously cloned biosynthetic genes involved in the production of the polyketide antibiotic actinorhodin by Streptomyces coelicolor A3(2). Complementation and mutational cloning analyses (in part using new phi C31 phage vectors incorporating a transcriptional terminator to block transcription from vector promoters into the cloned DNA) indicate that all the biosynthetic genes, including at least one regulatory (activator) gene, are clustered in a chromosomal region of about 26 kb. The genes are organised in at least four separate transcription units, ranging in size from 1 kb for the class III gene, to a polycistronic transcript of at least 5 kb for the class I, VII and IV genes. Indirect evidence shows that resistance to actinorhodin is also determined by the cloned DNA.

MeSH Terms
Anthraquinones/biosynthesis Anti-Bacterial Agents/biosynthesis Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics Genes, Bacterial Genetic Vectors Protein Biosynthesis Streptomyces/genetics Transcription, Genetic
Chemicals
Anthraquinones Anti-Bacterial Agents DNA Restriction Enzymes actinorhodin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Malpartida F
Hopwood D A
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26 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF02428033
Published
1986-10-00
Pages
66-73
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02428033
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