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PMID: 10742275 Published · ppublish English Comparative Study Journal Article

Method for rapid purification of class IIa bacteriocins and comparison of their activities.

Applied and environmental microbiology ·Vol. 66 ·No. 4 ·2000-04-00 ·Pages 1744-8

Guyonnet D, Fremaux C, Cenatiempo Y, Berjeaud JM

Abstract

A three-step method was developed for the purification of mesentericin Y105 (60% yield) from the culture supernatant of Leuconostoc mesenteroides Y105. The same procedure was successfully applied to the purification of five other anti-Listeria bacteriocins identified by mass spectrometry. Specific activities of the purified bacteriocins were compared.

MeSH Terms
Amino Acid Sequence Bacteriocins/chemistry,isolation & purification,pharmacology Bacteriological Techniques Chromatography, High Pressure Liquid Culture Media/chemistry Leuconostoc/growth & development,metabolism Listeria/drug effects Mass Spectrometry Molecular Sequence Data Sequence Alignment
Chemicals
Bacteriocins Culture Media
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Guyonnet D
IFR-CNRS, FR 59, "Communication Cellulaire,", France.
Fremaux C
Cenatiempo Y
Berjeaud J M
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.66.4.1744-1748.2000
Published
2000-04-00
Pages
1744-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC92056
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.66.4.1744-1748.2000 PMC Full Text View journal details
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